Customer reviews and FAQs

概述

  • 产品名称Anti-Caspase-12抗体
    参阅全部 Caspase-12 一抗
  • 描述
    兔多克隆抗体to Caspase-12
  • 特异性Recognises full-length and cleaved fragment of Caspase-12
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Human
  • 免疫原

    Synthetic peptide corresponding to Mouse Caspase-12.
    Database link: Q6UXS9
    (Peptide available as ab38274)

  • 阳性对照
    • Spleen tissue lysate

性能

应用

Our Abpromise guarantee covers the use of ab18766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 0.5 - 4 µg/ml. Detects procaspase 12 (55 kDa) and cleavage caspase 12 (36 kDa).
ICC/IF Use a concentration of 1 µg/ml.

靶标

Anti-Caspase-12 antibody 图像

  • Western blot - Anti-Caspase-12 antibody (ab18766)
    Western blot - Anti-Caspase-12 antibody (ab18766)
    Anti-Caspase-12 antibody (ab18766) + Mouse spleen tissue lysate
  • Western blot - Anti-Caspase-12 antibody (ab18766)
    Western blot - Anti-Caspase-12 antibody (ab18766)
    Anti-Caspase-12 antibody (ab18766) + Rat kidney tissue lysate
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-12 antibody (ab18766)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-12 antibody (ab18766)

    ab18766 (1µg/ml) staining caspase-12 in human liver using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cytoplasm and nuclei in hepatic arteries and plates of hepatic cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

  • Immunocytochemistry/ Immunofluorescence - Anti-Caspase-12 antibody (ab18766)
    Immunocytochemistry/ Immunofluorescence - Anti-Caspase-12 antibody (ab18766)

    ICC/IF image of ab18766 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18766, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1:1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Caspase-12 antibody (ab18766)参考文献

This product has been referenced in:
  • Crespo I  et al. Glutamine Treatment Attenuates Endoplasmic Reticulum Stress and Apoptosis in TNBS-Induced Colitis. PLoS One 7:e50407 (2012). WB ; Rat . Read more (PubMed: 23209735) »
  • Yang X  et al. Neurodegenerative and Inflammatory Pathway Components Linked to TNF-a/TNFR1 Signaling in the Glaucomatous Human Retina. Invest Ophthalmol Vis Sci 52:8442-54 (2011). WB ; Human . Read more (PubMed: 21917936) »

See all 4 Publications for this product

发表研究结果有使用 ab18766？请让我们知道，以便我们可以引用本数据表中的参考文章。

Product Wall

提交 ab18766 的 abreview 提交 ab18766 的问题

8 Abreviews or Q&A

10
Votes: Highest First

Thank you very much for your helpful answer.　Can I just ask you another question about the immunogen peptide ab38276 for　the antibody ab18766?　It is not indicated in the datasheet what is the exact sequence for the　peptide, how long it is, like it has ...

Read More

Thank you for your enquiry. Unfortunately, the exact immunogen sequence for the ab38276 peptide is proprietary and can not be released. The supplier was kind enough to inform us that the sequence is located around the 310 a.a. however any further i...

Read More

I purchased recently the anti-caspase 12 antibody ab18766 and I have a few questions for you. Thank you in advance for your attention. I am a bit confused about the epitope of this antibody. Indeed, in the data sheet, it is indicated that the syntheti...

Read More

I sincerely apologize for the wait. I finally manage to get some confirmation from the originator of the product regarding the immunogen sequence. I can confirm that the immunogen sequence for this ab18766 is located near the 310 amino acid, and th...

Read More

BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER 289777 DESCRIPTION OF THE PROBLEM Multiple bands SAMPLE Cytoplasmic extracts of human oligodendroglioma cells. PRIMARY ANTIBODY Caspase 12 rabbit polyclonal antibody from Abcam at...

Read More

Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab18766 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the ...

Read More

Thank you for your reply.I want to kown whether this primary antibody (ab18766) can detect tow style protein(pro caspase12 and cleaved caspase 12) or not. thanks.

Thank you for your enquiry. I can confirm that this product will recognize the full-length and cleaved fragment of Caspase 12. Unfortunately, we do not have any info on whether this product can also detect the pro caspase 12 form. In order to detec...

Read More

BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM wrong band size SAMPLE HeLa cell PRIMARY ANTIBODY Caspase 12 antibody / Rabbit / 0.5?/? in 5% skim milk / 1hr RT / 3 time at 5 min ...

Read More

Thank you for your enquiry. The antibody can detect ~50 kDa unprocessed protein, and also the cleaved fragment at around 30-40 kDa. You may not see the unprocessed precursor protein in all samples, since the protein will be processed and cleaved be...

Read More

About the anti-caspase-12 ab18766, what a pity it is that you don't have any western blot image for me to consult. Images will be valuable for consumers to make their decision. We are really interested in this product of your company. However, th...

Read More

Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you d...

Read More

I really appreciate your answer to my enquiry, but I am afraid that I have to bother you again. Do you have some Western blot images for caspase-12 antibody ab18766, to show me the specificity and species reactivity of this production? Th...

Read More

Thank you for your email. Unfortunately we do not have a Western blot image available at this time. Please rest assured that we guarantee that ab18766 will work in Western blotting. Our policy at Abcam is that if an antibody does not work as specif...

Read More

we are intreasted in your poduction caspase-12 antibody(ab18766), We want to know if you can help us to get some relative reasearch articles, which used this antibody to invesigate the fuction of caspase-12? I want to know if it 's possible to identify...

Read More

Thank you for your enquiry. Ab18766 recognizes full-length and cleaved fragment of Caspase 12. We are currently not aware of any publications featuring the use of this antibody. If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"