Caspase 12 (active) FITC Staining试剂盒(ab65610)

概述

  • 产品名称Caspase 12 (active) FITC Staining试剂盒
    参阅全部 Caspase 12 试剂盒
  • 检测类型Enzyme activity
  • 产品概述

    The Caspase 12 (active) FITC Staining Kit provides a convenient means for sensitive detection of activated caspase 12 in living cells. The assay utilizes the caspase 12 inhibitor, ATAD-FMK, conjugated to FITC (FITC-ATAD-FMK) as a marker. FITC-ATAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase 12 in apoptotic cells. The FITC label allows for direct detection of activated caspases in apoptotic cells by fluorescence microscopy, flow cytometry, or fluorescence plate reader.

  • 经测试应用适用于: Functional Studies, Flow Cytmore details

性能

  • 相关性Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. Caspase 12 is most highly related to members of the ICE subfamily. However, human caspase 12 has no protease activity. It may reduce cytokine release in response to bacterial lipopolysaccharide during infections and reduces activation of NF-kappa-B in response to TNF.
  • 别名
    • CASP12
    • CASP12P1
    • Caspase 12
    • Inactive caspase 12

相关产品

应用

Our Abpromise guarantee covers the use of ab65610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.

实验方案

Caspase 12 (active) FITC Staining Kit (ab65610)参考文献

This product has been referenced in:
  • Ryan SD  et al. Neuronal dystonin isoform 2 is a mediator of endoplasmic reticulum structure and function. Mol Biol Cell 23:553-66 (2012). Read more (PubMed: 22190742) »

See 1 Publication for this product

Product Wall

The developer of the kit suggests trypsinizing the cells and then using the protocol for cells in suspension. If you do the assay this way, it would probably be best any apply the apoptosis-inducing treatment to the cells after trypsinization, to the c...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"