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Abcam's Caspase 10 Assay Kit (Fluorometric) (ab65662) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence AEVD such as caspase 10. The assay is based on detection of cleavage of substrate AEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). AEVD-AFC emits blue light (lmax = 400 nm); upon cleavage of the substrate by caspase 10 or related caspases, free AFC emits a yellow-green fluorescence (lmax = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in AEVD-dependent caspase activity.
Store kit at –20oC. Store Cell Lysis Buffer and 2X Reaction Buffer at 4oC after opening. Protect AEVD-AFC from light. All reagents are stable for 6 months under proper storage conditions.
|2X Reaction Buffer||4 x 2ml|
|AEVD-AFC (1mM)||1 x 500µl|
|Cell Lysis Buffer||1 x 100ml|
|DTT||1 x 400µl|
Our Abpromise guarantee covers the use of ab65662 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|