Anti-Caspase-1 (phospho S376)抗体(ab61794)

概述

  • 产品名称Anti-Caspase-1 (phospho S376)抗体
    参阅全部 Caspase-1 一抗
  • 描述
    兔多克隆抗体to Caspase-1 (phospho S376)
  • 特异性ab61794 detects endogenous levels of Caspase-1 only when phosphorylated at serine 376.
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide corresponding to Human Caspase-1.
    Database link: P29466

  • 阳性对照
    • Human brain tissue and 293 cell extracts.
  • 常规说明

    The amino acid range of this immunogen is aa350-400 of human Caspase-1 with Acc# P29466 (http://www.uniprot.org/uniprot/P29466). This antibody binds to the Caspase-1 subunit p10 region. It binds to isoforms alpha, beta, gamma, delta, and epsilon.

性能

应用

Our Abpromise guarantee covers the use of ab61794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 45 kDa).

靶标

  • 功能Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
  • 组织特异性Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
  • 序列相似性Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • 翻译后修饰The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CASP-1 antibody
    • CASP1 antibody
    • CASP1_HUMAN antibody
    • Caspase 1 antibody
    • Caspase-1 subunit p10 antibody
    • ICE antibody
    • IL-1 beta-converting enzyme antibody
    • IL-1BC antibody
    • IL1 beta converting enzyme antibody
    • IL1B convertase antibody
    • Interleukin 1 beta convertase antibody
    • Interleukin 1B converting enzyme antibody
    • Interleukin-1 beta convertase antibody
    • Interleukin-1 beta-converting enzyme antibody
    • p45 antibody
    see all

Anti-Caspase-1 (phospho S376) antibody 图像

  • All lanes : Anti-Caspase-1 (phospho S376) antibody (ab61794) at 1/500 dilution

    Lane 1 : 293 cell extracts
    Lane 2 : 293 cell extracts with immunising peptide


    Predicted band size : 45 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • ab61794 at 1:50 - 1:100 dilution staining Caspase-1 in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

Anti-Caspase-1 (phospho S376) antibody (ab61794)参考文献

ab61794 has not yet been referenced specifically in any publications.

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Thank you for your email.

We do not have any experimental data regarding the reactivity of this antibody to the active or inactive form of Caspase 1. By sequence homology this antibody will recognize both the inactive and active forms of Caspa...

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Thank you for contacting Abcam.
Based on the information from UniPort (SwissProt: P29466), there are a number of isoforms of Caspase-1, one of which is about 30kDa. I am not sure which isoforms is expressed in 293 cell extracts.
Although we d...

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