重组
RabMAb

Anti-Caspase-1抗体[EPR4321] (HRP) (ab201476)

概述

  • 产品名称
    Anti-Caspase-1抗体[EPR4321] (HRP)
    参阅全部 Caspase-1 一抗
  • 描述
    兔单克隆抗体[EPR4321] to Caspase-1 (HRP)
  • 宿主
    Rabbit
  • 偶联物
    HRP
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Recombinant fragment corresponding to Human Caspase-1 aa 250 to the C-terminus (C terminal).
    Database link: P29466

  • 阳性对照
    • WB: HeLa, THP-1, Jurkat and C6 whole cell lysates
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab201476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

靶标

  • 功能
    Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
  • 组织特异性
    Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
  • 序列相似性
    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • 翻译后修饰
    The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CASP-1 antibody
    • CASP1 antibody
    • CASP1_HUMAN antibody
    • Caspase 1 antibody
    • Caspase-1 subunit p10 antibody
    • ICE antibody
    • IL-1 beta-converting enzyme antibody
    • IL-1BC antibody
    • IL1 beta converting enzyme antibody
    • IL1B convertase antibody
    • Interleukin 1 beta convertase antibody
    • Interleukin 1B converting enzyme antibody
    • Interleukin-1 beta convertase antibody
    • Interleukin-1 beta-converting enzyme antibody
    • p45 antibody
    see all

图片

  • All lanes : Anti-Caspase-1 antibody [EPR4321] (HRP) (ab201476) at 1/5000 dilution

    Lane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : C6 (Rat brain glioma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 20 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab201476 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

实验方案

文献

ab201476 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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