Anti-Caspase-1抗体[EPR4321] (ab108362)


  • 产品名称Anti-Caspase-1抗体[EPR4321]
    参阅全部 Caspase-1 一抗
  • 描述
    兔单克隆抗体[EPR4321] to Caspase-1
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: ICC or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment corresponding to Human Caspase-1 aa 250 to the C-terminus (C terminal).
    Database link: P29466

  • 阳性对照
    • THP-1, Jurkat, C6 and NIH/3T3 cell lysates Paraffin-embedded Human breast invasive ductal carcinoma tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Alternative versions available:

    Anti-Caspase-1 antibody (HRP) [EPR4321] (ab201476)

    Anti-Caspase-1 antibody (Biotin) [EPR4321] (ab201478)




Our Abpromise guarantee covers the use of ab108362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IHC-P 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for ICC or IP.
  • 靶标

    • 功能Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
    • 组织特异性Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
    • 序列相似性Belongs to the peptidase C14A family.
      Contains 1 CARD domain.
    • 翻译后修饰The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CASP-1 antibody
      • CASP1 antibody
      • CASP1_HUMAN antibody
      • Caspase 1 antibody
      • Caspase-1 subunit p10 antibody
      • ICE antibody
      • IL-1 beta-converting enzyme antibody
      • IL-1BC antibody
      • IL1 beta converting enzyme antibody
      • IL1B convertase antibody
      • Interleukin 1 beta convertase antibody
      • Interleukin 1B converting enzyme antibody
      • Interleukin-1 beta convertase antibody
      • Interleukin-1 beta-converting enzyme antibody
      • p45 antibody
      see all

    Anti-Caspase-1 antibody [EPR4321] 图像

    • All lanes : Anti-Caspase-1 antibody [EPR4321] (ab108362) at 1/10000 dilution (purified)

      Lane 1 : THP-1 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP goat anti-rabbit at 1/1000 dilution

      Predicted band size : 45 kDa

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemical staining of paraffin embedded human kidney with purified ab108362 at a dilution of 1/500. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    • All lanes : Anti-Caspase-1 antibody [EPR4321] (ab108362) at 1/1000 dilution (unpurified)

      Lane 1 : THP-1 cell lysates
      Lane 2 : THP-1+TPA+LPS cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : C6 cell lysate
      Lane 5 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 45 kDa
    • Immunohistochemical staining of caspase-1 in paraffin-embedded human breast invasive ductal carcinoma tissue with unpurified ab108362, at a dilution of 1/250.

    Anti-Caspase-1 antibody [EPR4321] (ab108362)参考文献

    This product has been referenced in:
    • Zhao J  et al. Synthetic Oligodeoxynucleotides Containing Multiple Telemeric TTAGGG Motifs Suppress Inflammasome Activity in Macrophages Subjected to Oxygen and Glucose Deprivation and Reduce Ischemic Brain Injury in Stroke-Prone Spontaneously Hypertensive Rats. PLoS One 10:e0140772 (2015). WB ; Rat . Read more (PubMed: 26473731) »
    • Zhang X  et al. Porcine Mx1 fused to HIV Tat protein transduction domain (PTD) inhibits classical swine fever virus infection in vitro and in vivo. BMC Vet Res 11:264 (2015). Pig . Read more (PubMed: 26472464) »

    See all 6 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (Hep G2)
    Specification Hep G2
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Abcam user community

    Verified customer

    提交于 Dec 30 2014

    The immunogen sequence that the antibody was generated against contains a portion of p20 and a portion of p10, but the single epitope that this monoclonal antibody recognizes has not been mapped to either of those portions. So, it should be able to rec...

    Read More

    Thank you for your email.

    This protein is ubiquotsly expressed in many organs including brain (http://www.fasebj.org/content/13/8/813.long) fig: 3 so finding a right negative control might be a problem. Please check the human protein atlas lin...

    Read More

    Thank you for your calls today, and I'm sorry we don't have a replacement immediately available for you. Your credit note ID is *** . If payment has already been made on the original order and you wish to receive a refund, please ask you...

    Read More