概述

  • 产品名称Anti-Caspase-1抗体
    参阅全部 Caspase-1 一抗
  • 描述
    兔多克隆抗体to Caspase-1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Pig
  • 免疫原

    Synthetic peptide corresponding to Human Caspase-1 aa 350 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P29466
    (Peptide available as ab91439, ab91439)

  • 阳性对照
    • This antibody gave a positive signal in Human spleen and lung tissue lysates as well as the following whole cell lysates: HeLa; A549; HepG2; CaCo2.

性能

应用

Our Abpromise guarantee covers the use of ab82563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

靶标

  • 功能Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
  • 组织特异性Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
  • 序列相似性Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • 翻译后修饰The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CASP-1 antibody
    • CASP1 antibody
    • CASP1_HUMAN antibody
    • Caspase 1 antibody
    • Caspase-1 subunit p10 antibody
    • ICE antibody
    • IL-1 beta-converting enzyme antibody
    • IL-1BC antibody
    • IL1 beta converting enzyme antibody
    • IL1B convertase antibody
    • Interleukin 1 beta convertase antibody
    • Interleukin 1B converting enzyme antibody
    • Interleukin-1 beta convertase antibody
    • Interleukin-1 beta-converting enzyme antibody
    • p45 antibody
    see all

Anti-Caspase-1 antibody 图像

  • All lanes : Anti-Caspase-1 antibody (ab82563) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)
    Lane 3 : Lung (Human) Tissue Lysate
    Lane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
    Additional bands at : 50 kDa,87 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes

Anti-Caspase-1 antibody (ab82563)参考文献

ab82563 has not yet been referenced specifically in any publications.

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