1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
功能Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
组织特异性Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
序列相似性Belongs to the peptidase C14A family. Contains 1 CARD domain.
翻译后修饰The two subunits are derived from the precursor sequence by an autocatalytic mechanism.