The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 20 µg/ml.
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
Use at an assay dependent concentration.
功能Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
组织特异性Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
序列相似性Belongs to the peptidase C14A family. Contains 1 CARD domain.
翻译后修饰The two subunits are derived from the precursor sequence by an autocatalytic mechanism.