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Our Abpromise guarantee covers the use of ab1872 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. PubMed: 21228274|
ab1872 staining Caspase-1 in mouse immortalised bone marrow-derived macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 (0.1%) and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1:200 in PBS + 0.1% BSA/5% goat serum) for 1 hour at 20°C. An anti-rabbit Alexa Fluor®568 (1/500) ab175471) was used as the secondary antibody.
ab1872 staining Caspase-1 in formalin-fixed, paraffin-embedded Human placenta tissue by Immunohistochemistry.
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