Anti-Caspase-1抗体(ab138483)
Key features and details
- Rabbit polyclonal to Caspase-1
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Caspase-1抗体
参阅全部 Caspase-1 一抗 -
描述
兔多克隆抗体to Caspase-1 -
宿主
Rabbit -
特异性
Replenishment batches of ab138483 are tested in WB. Previous batches were additionally validated in IHC-P. This application is still expected to work and is covered by our Abpromise guarantee.
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经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide corresponding to Mouse Caspase-1 aa 100-200 conjugated to keyhole limpet haemocyanin.
Database link: P29452 -
阳性对照
- This antibody gave a positive signal in Mouse Spleen tissue lysate IHC-P: FFPE mouse lung tissue sections.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab138483于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 45 kDa).
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IHC-P | (1) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 45 kDa). |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. -
组织特异性
Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. -
序列相似性
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
翻译后修饰
The two subunits are derived from the precursor sequence by an autocatalytic mechanism. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 12362 Mouse
- SwissProt: P29452 Mouse
- Unigene: 1051 Mouse
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别名
- CASP-1 antibody
- CASP1 antibody
- CASP1_HUMAN antibody
see all
图片
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Anti-Caspase-1 antibody (ab138483) at 1 µg/ml (Milk blocking 3%) + Spleen (Mouse) Tissue Lysate at 25 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Additional bands at: 74 kDa (possible non-specific binding)
Exposure time: 20 minutesThe predicted molecular weight of Caspase 1 is 45 kDa (SwissProt), however we expect to observe a banding pattern around 50 kDa.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab138483 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
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IHC image of Caspase-1 staining in mouse lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab138483, 1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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SDS download
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Datasheet download
文献 (44)
ab138483 被引用在 44 文献中.
- Sun J et al. USF2 knockdown downregulates THBS1 to inhibit the TGF-β signaling pathway and reduce pyroptosis in sepsis-induced acute kidney injury. Pharmacol Res 176:105962 (2022). PubMed: 34756923
- Liu C et al. Liquiritin Alleviates Depression-Like Behavior in CUMS Mice by Inhibiting Oxidative Stress and NLRP3 Inflammasome in Hippocampus. Evid Based Complement Alternat Med 2022:7558825 (2022). PubMed: 35069768
- Li G et al. miR-155 inhibits chondrocyte pyroptosis in knee osteoarthritis by targeting SMAD2 and inhibiting the NLRP3/Caspase-1 pathway. J Orthop Surg Res 17:48 (2022). PubMed: 35090521
- Liu Q et al. Thioredoxin reductase 3 suppression promotes colitis and carcinogenesis via activating pyroptosis and necrosis. Cell Mol Life Sci 79:106 (2022). PubMed: 35094204
- Shen J et al. TLR9 regulates NLRP3 inflammasome activation via the NF-kB signaling pathway in diabetic nephropathy. Diabetol Metab Syndr 14:26 (2022). PubMed: 35120573