特异性By Western blot, this antibody detects a 112 kDa protein representing CASK from AtT20 cell lysate.
Immunohistochemical staining of CASK in mouse brain with this antibody results in staining of the choroid plexus.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 µg/ml. Predicted molecular weight: 105 kDa.Can be blocked with CASK peptide (ab4961).
功能Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1.
组织特异性Ubiquitous. Expression is significantly greater in brain relative to kidney, lung, and liver and in fetal brain and kidney relative to lung and liver.
疾病相关Defects in CASK are the cause of mental retardation X-linked CASK-related (MRXCASK) [MIM:300749]. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Patients with mental retardation X-linked CASK-related can manifest a severe phenotype consisting of severe intellectual deficit, congenital or postnatal microcephaly, disproportionate brainstem and cerebellar hypoplasia (MICPCH Syndrome). A milder phenotype consists of mental retardation alone or associated with nystagmus. Defects in CASK are the cause of FG syndrome type 4 (FGS4) [MIM:300422]. FG syndrome (FGS) is an X-linked disorder characterized by mental retardation, relative macrocephaly, hypotonia and constipation.
序列相似性In the N-terminal section; belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Belongs to the MAGUK family. Contains 1 guanylate kinase-like domain. Contains 2 L27 domains. Contains 1 PDZ (DHR) domain. Contains 1 protein kinase domain. Contains 1 SH3 domain.
结构域The first L27 domain binds DLG1 and the second L27 domain probably binds LIN7. The protein kinase domain mediates the interaction with FCHSD2.
Calcium/calmodulin dependent serine protein kinase antibody
Calcium/calmodulin dependent serine protein kinase (MAGUK family) antibody
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LIN 2 antibody
Lin 2 homolog antibody
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Protein lin-2 homolog antibody
Trinucleotide repeat containing 8 antibody
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Anti-CASK antibody 图像
Western blot - Anti-CASK antibody (ab3383)
Predicted band size : 105 kDa
Western blot detection of CASK on AtT20 cell lysate using ab3383.
Anti-CASK antibody (ab3383)参考文献
This product has been referenced in:
Ojeh N et al. The MAGUK-family protein CASK is targeted to nuclei of the basal epidermis and controls keratinocyte proliferation. J Cell Sci121:2705-17 (2008).
Read more (PubMed: 18664494) »