Anti-Casein Kinase 2 beta (phospho S209)抗体(ab12861)

概述

  • 产品名称Anti-Casein Kinase 2 beta (phospho S209)抗体
    参阅全部 Casein Kinase 2 beta 一抗
  • 描述
    兔多克隆抗体to Casein Kinase 2 beta (phospho S209)
  • 特异性Does not cross react with Casein Kinase 2 alpha [pS360] (mouse), [pS362], [pS370], or Casein Kinase 2 beta pS2].
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic phosphopeptide (Human) from the region of casein kinase 2 that contains serine 209.

  • 阳性对照
    • HeLa cell lysates or K562 cell lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 1mg/ml BSA
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Casein Kinase 2 beta. The final product is generated by affinity chromatography using a Casein Kinase 2 beta derived peptide that is phosphorylated at serine 209.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab12861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/1000 in 3% milk in 0.1% Tween20 (TBST). Incubate overnight. Detects a band of approximately 25 kDa. Block 1hr with 3% milk in 0.1% Tween20 (TBST).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Participates in Wnt signaling (By similarity). Plays a complex role in regulating the basal catalytic activity of the alpha subunit.
    • 序列相似性Belongs to the casein kinase 2 subunit beta family.
    • 翻译后修饰Phosphorylated by alpha subunit.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Casein kinase 2 beta polypeptide antibody
      • Casein kinase II beta subunit antibody
      • Casein kinase II subunit beta antibody
      • CK II beta antibody
      • CK2B antibody
      • CK2N antibody
      • CSK2B antibody
      • CSK2B_HUMAN antibody
      • CSNK 2B antibody
      • csnk2b antibody
      • G5A antibody
      • MGC138222 antibody
      • MGC138224 antibody
      • Phosvitin antibody
      • Protein G5a antibody
      see all

    Anti-Casein Kinase 2 beta (phospho S209) antibody 图像

    • Peptide Competition and Phosphatase Treatment

      Lysates prepared from HeLa cells were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda (λ) phosphatase (5), blocked with a 3% Milk-TBST buffer for one hour at room temperature, and incubated with CK2β [pS209] (ab12861) antibody for two hours at room temperature in a 3% Milk- TBST buffer, following prior incubation with: no peptide (1, 5), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphoserine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method.

      The data show that only the peptide corresponding to CK2β [pS209] blocks the signal, verifying the specificity of the antibody.

    Anti-Casein Kinase 2 beta (phospho S209) antibody (ab12861)参考文献

    ab12861 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry. The S209 refers to serine 209. The immunogen used to generate this antibody was a synthetic phosphopeptide (Human) from the region of casein kinase 2 that contains serine 209. The antibody has been negatively preadsorbed...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"