Anti-Casein Kinase 2 beta抗体[EP1995Y] (ab76025)


  • 产品名称Anti-Casein Kinase 2 beta抗体[EP1995Y]
    参阅全部 Casein Kinase 2 beta 一抗
  • 描述
    兔单克隆抗体[EP1995Y] to Casein Kinase 2 beta
  • 经测试应用适用于: WB, Flow Cyt, IHC-P, ICC/IFmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Horse, Human
  • 免疫原

    Synthetic peptide corresponding to residues near the C terminus of human Casein Kinase 2 beta protein

  • 阳性对照
    • WB: K562 cell lysate ICC/IF: HeLa cells IHC-P: Human ovary carcinoma tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-Casein Kinase 2 beta antibody (HRP) [EP1995Y] (ab200789)



Our Abpromise guarantee covers the use of ab76025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 25 kDa.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 100 - 250 mg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for IP.
  • 靶标

    Anti-Casein Kinase 2 beta antibody [EP1995Y] 图像

    • Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025) at 1/1000 dilution + K562 cell lysate at 10 µg

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 25 kDa
      Observed band size : 25 kDa
    • Immunofluorescent staining of Casein Kinase 2 beta in HeLa cells using ab76025 at a 1/100 dilution.
    • Immunohistochemical analysis of Casein Kinase 2 beta in paraffin embedded human ovary carcinoma tissue using ab76025 at a 1/100 dilution.
    • Overlay histogram showing HeLa cells stained with ab76025 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76025, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)参考文献

    ab76025 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (C57BL/6 Mouse splenocytes)
    Loading amount 40 µg
    Specification C57BL/6 Mouse splenocytes
    Gel Running Conditions Reduced Denaturing (10% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Dr. Tomar Ghansah

    Verified customer

    提交于 Oct 05 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Horse Tissue sections (Mammary gland tissue)
    Specification Mammary gland tissue
    Fixative Formaldehyde
    Antigen retrieval step None
    Permeabilization No

    Dr. Jan Spaas

    Verified customer

    提交于 Jun 09 2011