重组
RabMAb

Anti-Casein Kinase 2 beta抗体[EP1995Y] (ab76025)

概述

  • 产品名称
    Anti-Casein Kinase 2 beta抗体[EP1995Y]
    参阅全部 Casein Kinase 2 beta 一抗
  • 描述
    兔单克隆抗体[EP1995Y] to Casein Kinase 2 beta
  • 经测试应用
    适用于: WB, Flow Cyt, IHC-P, ICC/IFmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Horse, Human
  • 免疫原

    Synthetic peptide corresponding to residues near the C terminus of human Casein Kinase 2 beta protein

  • 阳性对照
    • WB: K562 cell lysate ICC/IF: HeLa cells IHC-P: Human ovary carcinoma tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Alternative versions available:

    Anti-Casein Kinase 2 beta antibody (HRP) [EP1995Y] (ab200789)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab76025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 25 kDa.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 100 - 250 mg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • 应用说明
    Is unsuitable for IP.
  • 靶标

    图片

    • Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025) at 1/1000 dilution + K562 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 25 kDa
      Observed band size : 25 kDa
    • Immunofluorescent staining of Casein Kinase 2 beta in HeLa cells using ab76025 at a 1/100 dilution.
    • Immunohistochemical analysis of Casein Kinase 2 beta in paraffin embedded human ovary carcinoma tissue using ab76025 at a 1/100 dilution.
    • Overlay histogram showing HeLa cells stained with ab76025 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76025, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    • Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025) at 1/1000 dilution + MEF whole cell lysate at 20 µg

      Secondary
      Alexa Fluor® 680-conjugated polyclonal goat anti-rabbit IgG at 1/5000 dilution

      Performed under reducing conditions.

      Predicted band size : 25 kDa
      Observed band size : 25 kDa


      Exposure time : 10 minutes

      This image is courtesy of an anonymous Abreview

      Blocked for 30 minutes at 25°C.

      Incubated with the primary antibody for 2 hours at 25°C in blocking buffer.

      See Abreview

    文献

    ab76025 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (U2OS cells)
    Total protein in input
    500 µg
    Immuno-precipitation step
    Protein A/G
    Specification
    U2OS cells
    Username

    Abcam user community

    Verified customer

    提交于 May 12 2017

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (MEF cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Gradient Gel)
    Loading amount
    20 µg
    Specification
    MEF cells
    Blocking step
    Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 May 03 2017

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Gradient Gel)
    Loading amount
    50 µg
    Specification
    U2OS cells
    Blocking step
    Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 16 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (C57BL/6 Mouse splenocytes)
    Loading amount
    40 µg
    Specification
    C57BL/6 Mouse splenocytes
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Tomar Ghansah

    Verified customer

    提交于 Oct 05 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Horse Tissue sections (Mammary gland tissue)
    Specification
    Mammary gland tissue
    Fixative
    Formaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Username

    Dr. Jan Spaas

    Verified customer

    提交于 Jun 09 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册