Anti-Casein Kinase 1 gamma 2抗体(ab102554)

概述

  • 产品名称Anti-Casein Kinase 1 gamma 2抗体
    参阅全部 Casein Kinase 1 gamma 2 一抗
  • 描述
    兔多克隆抗体to Casein Kinase 1 gamma 2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 359-408 (SKNQALNSTN GELNADDPTA GHSNAPITAP AEVEVADETK CCCFFKRRKR) of Human Casein Kinase 1 gamma 2 (NP_001310).

  • 阳性对照
    • A549 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab102554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity). Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis.
  • 组织特异性Testis.
  • 序列相似性Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Casein kinase 1, gamma 2 antibody
    • Casein kinase I isoform gamma 2 antibody
    • Casein kinase I isoform gamma-2 antibody
    • CK1g2 antibody
    • CKI gamma 2 antibody
    • CKI-gamma 2 antibody
    • CSNK1G 2 antibody
    • Csnk1g2 antibody
    • KC1G2_HUMAN antibody
    see all

Anti-Casein Kinase 1 gamma 2 antibody 图像

  • Anti-Casein Kinase 1 gamma 2 antibody (ab102554) at 1 µg/ml + A549 cell lysate at 10 µg

    Predicted band size : 47 kDa

Anti-Casein Kinase 1 gamma 2 antibody (ab102554)参考文献

ab102554 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human osteosarcoma U2OS cells)
Loading amount 90 µg
Specification Human osteosarcoma U2OS cells
Gel Running Conditions Reduced Denaturing (10%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Apr 12 2013

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