Anti-Casein Kinase 1 alpha (phospho T1194)抗体(ab63559)

概述

  • 产品名称Anti-Casein Kinase 1 alpha (phospho T1194)抗体
    参阅全部 Casein Kinase 1 alpha 一抗
  • 描述
    兔多克隆抗体to Casein Kinase 1 alpha (phospho T1194)
  • 特异性Detects Casein Kinase I alpha only when phosphorylated at threonine 321.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human Casein Kinase I around the phosphorylation site of threonine 321 (A-Q-TP-P-T).

  • 阳性对照
    • HuvEc cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab63559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
ELISA 1/10000.

靶标

  • 功能Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.
  • 序列相似性Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
    Contains 1 protein kinase domain.
  • 细胞定位Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Nucleus speckle. Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Casein kinase 1 alpha 1 antibody
    • Casein kinase I isoform alpha antibody
    • CK1 antibody
    • CK1A antibody
    • CKI alpha antibody
    • CKI-alpha antibody
    • CKIa antibody
    • Clock regulator kinase antibody
    • Csnk1a1 antibody
    • Down regulated in lung cancer antibody
    • HLCDGP1 antibody
    • KC1A_HUMAN antibody
    • PRO2975 antibody
    see all

Anti-Casein Kinase 1 alpha (phospho T1194) antibody 图像

  • All lanes : Anti-Casein Kinase 1 alpha (phospho T1194) antibody (ab63559) at 1/500 dilution

    Lane 1 : extracts from HuvEc cells
    Lane 2 : extracts from HuvEc cells with immunizing phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 39 kDa
    Observed band size : 39 kDa

Anti-Casein Kinase 1 alpha (phospho T1194) antibody (ab63559)参考文献

ab63559 has not yet been referenced specifically in any publications.

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