RabMAb

Anti-Casein Kinase 1 alpha抗体[EPR1961(2)] (ab108296)

概述

  • 产品名称Anti-Casein Kinase 1 alpha抗体[EPR1961(2)]
    参阅全部 Casein Kinase 1 alpha 一抗
  • 描述
    兔单克隆抗体[EPR1961(2)] to Casein Kinase 1 alpha
  • 经测试应用适用于: WBmore details
    不适用于: Flow Cyt,ICC,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Casein Kinase 1 alpha aa 250-350.

  • 阳性对照
    • MCF-7, HeLa, Jurkat, and HT-1376 cell lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab108296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 39 kDa).
  • 应用说明Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • 靶标

    • 功能Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.
    • 序列相似性Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
      Contains 1 protein kinase domain.
    • 细胞定位Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Nucleus speckle. Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Casein kinase 1 alpha 1 antibody
      • Casein kinase I isoform alpha antibody
      • CK1 antibody
      • CK1A antibody
      • CKI alpha antibody
      • CKI-alpha antibody
      • CKIa antibody
      • Clock regulator kinase antibody
      • Csnk1a1 antibody
      • Down regulated in lung cancer antibody
      • HLCDGP1 antibody
      • KC1A_HUMAN antibody
      • PRO2975 antibody
      see all

    Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] 图像

    • All lanes : Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : HT-1376 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 39 kDa
      Observed band size : 34 kDa (why is the actual band size different from the predicted?)

    Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)参考文献

    This product has been referenced in:
    • Zemp I  et al. CK1d and CK1e are components of human 40S subunit precursors required for cytoplasmic 40S maturation. J Cell Sci 127:1242-53 (2014). WB . Read more (PubMed: 24424021) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (lymphoid cell)
    Gel Running Conditions Reduced Denaturing (10 % gel)
    Loading amount 10 µg
    Specification lymphoid cell
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    提交于 Nov 18 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"