参阅全部 CARS 一抗
WB, ELISAmore details
Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
Synthetic peptide designed within internal sequence amino acids 755-804: QEQEAAKLAK MKIPPSEMFL SETDKYSKFD ENGLPTHDME GKELSKGQAK, of Human CARS (NP_001014437)
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- 存储溶液Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
- 纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 86 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
Titre using peptide based assay: 1/1562500.
- 疾病相关Note=A chromosomal aberration involving CARS is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with ALK.
- 序列相似性Belongs to the class-I aminoacyl-tRNA synthetase family.
Information by UniProt
- Cysteine tRNA ligase antibody
- Cars antibody
- CysRS antibody
Anti-CARS antibody 图像
Western blot - CARS antibody (ab83256)
Anti-CARS antibody (ab83256) at 1 µg/ml (in 5% skim milk/ PBS) + MCF7 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 86 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
Anti-CARS antibody (ab83256)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"