Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
Synthetic peptide designed within internal sequence amino acids 755-804: QEQEAAKLAK MKIPPSEMFL SETDKYSKFD ENGLPTHDME GKELSKGQAK, of Human CARS (NP_001014437)
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 86 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
Titre using peptide based assay: 1/1562500.
Note=A chromosomal aberration involving CARS is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with ALK.
Belongs to the class-I aminoacyl-tRNA synthetase family.
Information by UniProt
- Cysteine tRNA ligase antibody
- Cars antibody
- CysRS antibody
Western blot - CARS antibody (ab83256)
Anti-CARS antibody (ab83256) at 1 µg/ml (in 5% skim milk/ PBS) + MCF7 cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size :
86 kDaObserved band size :
95 kDa (why is the actual band size different from the predicted?
)Additional bands at :
40 kDa. We are unsure as to the identity of these extra bands.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"