兔多克隆抗体to Cardiotrophin 1
Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Cardiotrophin 1.
(Peptide available as
This antibody gave a positive signal in HepG2 whole cell lysate as well as Cardiotrophin 1 active recombinant protein.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa).
Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor.
Highly expressed in heart, skeletal muscle, prostate and ovary. Lower levels in lung, kidney, pancreas, thymus, testis and small intestine. Little or no expression in brain, placenta, liver, spleen, colon or peripheral blood leukocytes.
Belongs to the IL-6 superfamily.
Information by UniProt
Cardiophin 1 antibody
Cardiotrophin I antibody
Western blot - Anti-Cardiotrophin 1 antibody (ab83176)
All lanes :
Anti-Cardiotrophin 1 antibody (ab83176) at 1 µg/ml
Lane 1 :
HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2 : Recombinant human Cardiotrophin 1 protein ( ab9838) at 0.1 µg Secondary All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa Observed band size: 24 kDa ( why is the actual band size different from the predicted?) Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands. Exposure time: 30 seconds
has not yet been referenced specifically in any publications.
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