Anti-Cardiotin抗体[R2G] (ab8962)
Key features and details
- Mouse monoclonal [R2G] to Cardiotin
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig, Zebrafish
- Isotype: IgM
概述
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产品名称
Anti-Cardiotin抗体[R2G] -
描述
小鼠单克隆抗体[R2G] to Cardiotin -
宿主
Mouse -
特异性
This antibody reacts with cardiotin, a mitochondrion-associated protein which is present in cardiomyocytes and skeletal muscle. -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human, Pig, Zebrafish -
免疫原
Full length native protein (purified) corresponding to Chicken Cardiotin. Total protein extract of chicken gizzard
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常规说明
Mitochondrial membrane proteins have been shown to be involved in several cellular processes such as the regulation of apoptosis and the energy metabolism. Cardiotin antibodies, which are reactive with mitochondrial proteins, have proven to be early indicators of ischemia induced changes in the myocardium.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
单克隆 -
克隆编号
R2G -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgM -
研究领域
相关产品
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Compatible Secondaries
应用
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration.
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WB |
1/25 - 1/500.
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说明 |
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IHC-P
Use at an assay dependent concentration. |
WB
1/25 - 1/500. |
靶标
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相关性
Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondrial membrane. The cardiotin structure exists as subunits of 60 kDa and 100 kDa. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle a-actinin, suggesting that the tetrameric configuration of the cardiotin protein structure is a transmembrane complex with the N-terminus at the cytoplasmic side of the membrane, able to interact with actin. During cardiac contractile dysfunction, the cardiotin distribution is affected in pathlogical cardiomyocytes i.e. chronic ischemic myocardium. This monoclonal antibody can be used in immunohistochemistry for the detection of a disturbed mitochondrial activity in cardiomyocytes i.e. during chronic ischemia or chronic atrial fibrillation.
图片
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Indirect immunofluorescence analysis of swine heart cells labelling cardiotin with ab8962.
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Indirect immunofluorescence analysis of swine heart cells labelling cardiotin with ab8962.
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Human atrial cardiomyocytes stained with the monoclonal cardiotin antibody R2G.
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Immunoblotting of the microsomal fraction of swine heart reveals the 100 kDa and 60 kDa cardiotin subunits after incubation with monoclonal antibodies (a) ab8963 clone SR-2, (b) ab8964 clone SR-3 and (c) ab8962 clone R2G.
Immunoblotting of the microsomal fraction of swine heart reveals the 100 kDa and 60 kDa cardiotin subunits after incubation with monoclonal antibodies (a) ab8963 clone SR-2, (b) ab8964 clone SR-3 and (c) ab8962 clone R2G. -
Immunofluorescence staining of a 7 days old zebrafish embryo
数据表及文件
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Datasheet download
文献 (2)
ab8962 被引用在 2 文献中.
- Talavera-Adame D et al. Enhancement of embryonic stem cell differentiation promoted by avian chorioallantoic membranes. Tissue Eng Part A 15:3193-200 (2009). PubMed: 19364272
- Dispersyn GD et al. Dissociation of cardiomyocyte apoptosis and dedifferentiation in infarct border zones. Eur Heart J 23:849-57 (2002). PubMed: 12042006