Anti-Cardioactive肽抗体(ab58736)

概述

  • 产品名称
    Anti-Cardioactive肽抗体
  • 描述
    兔多克隆抗体to Cardioactive肽
  • 经测试应用
    适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Recombinant fragment
    预测可用于: Drosophila melanogaster
  • 免疫原

    Synthetic peptide corresponding to N-terminal residues of Drosophila melanogaster Cardioactive Peptide

性能

应用

Our Abpromise guarantee covers the use of ab58736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 18 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.
ICC/IF Use at an assay dependent concentration.

靶标

  • 相关性
    Cardioactive peptide (CCAP) is a cardioregulatory neurohormone that increases heart beat rate during adult wing inflation. This protein has no effect on beat amplitude. The effect of cardioactive peptide is both ino- and conotrophic. Cardioactive peptide has tissue specificity within the central nervous system and most neurons exhibit coexpression with burs.
  • 细胞定位
    Secreted
  • 数据库链接
    • 别名
      • Cardioacceleratory peptide 2a antibody
      • CCAP antibody
      • CG4910 antibody
      • Crustacean cardioactive peptide antibody
      • DmCCAP antibody
      see all

    图片

    • Drosophila third instar larvae were dissected and fixed to get a fillet preparation. Samples were fixed in formaldehyde, permeabilized using Triton X-100, blocked with 5% serum for 1 hour at 25°C and then incubated with ab58736 at a 1/500 dilution. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.
      Cardioactive Peptide shows positive staining colocalizing with neuronal marker for HRP.

      See Abreview

    文献

    ab58736 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Fruit fly (Drosophila melanogaster) Cell (neuromuscular junction of drosophila larvae)
    Specification
    neuromuscular junction of drosophila larvae
    Fixative
    Formaldehyde
    Permeabilization
    Yes - triton x100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    提交于 Dec 28 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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