RabMAb

Anti-Cardiac Troponin T抗体[EPR3695] (ab91605)

概述

  • 产品名称Anti-Cardiac Troponin T抗体[EPR3695]
    参阅全部 Cardiac Troponin T 一抗
  • 描述
    兔单克隆抗体[EPR3695] to Cardiac Troponin T
  • 经测试应用适用于: WB, IP, IHC-Pmore details
    不适用于: Flow Cyt or ICC
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues within Human Cardiac Troponin T

  • 阳性对照
    • WB: Human cardiac tissue lysate. IHC-P: Human cardiac muscle tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab91605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100000 - 1/500000. Predicted molecular weight: 36 kDa.
IP 1/10.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

The use of an HRP/AP polymerized secondary antibody is recommended as stronger signals have been found using these. IHC-P validated in Human samples only.

  • 应用说明Is unsuitable for Flow Cyt or ICC.
  • 靶标

    • 功能Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
    • 组织特异性Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
    • 疾病相关Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
      Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
      Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
    • 序列相似性Belongs to the troponin T family.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cardiac muscle troponin T antibody
      • Cardiomyopathy dilated 1D (autosomal dominant) antibody
      • Cardiomyopathy hypertrophic 2 antibody
      • CMD1D antibody
      • CMH2 antibody
      • CMPD2 antibody
      • cTnT antibody
      • LVNC6 antibody
      • MGC3889 antibody
      • OTTHUMP00000033864 antibody
      • OTTHUMP00000033865 antibody
      • OTTHUMP00000033866 antibody
      • OTTHUMP00000033867 antibody
      • OTTHUMP00000033870 antibody
      • OTTHUMP00000218095 antibody
      • RCM3 antibody
      • TNNT 2 antibody
      • TNNT2 antibody
      • TNNT2_HUMAN antibody
      • TnTc antibody
      • Troponin T cardiac muscle antibody
      • Troponin T type 2 (cardiac) antibody
      • Troponin T type 2 cardiac antibody
      • Troponin T, cardiac muscle antibody
      • Troponin T2 cardiac antibody
      see all

    Anti-Cardiac Troponin T antibody [EPR3695] 图像

    • All lanes : Anti-Cardiac Troponin T antibody [EPR3695] (ab91605) at 1/50000 dilution

      Lane 1 : Human heart tissue lysate
      Lane 2 : Human skeletal muscle tissue lysate
      Lane 3 : 293T whole cell lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 36 kDa
      Observed band size : 39 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 second

      Blocking and dilution buffer: 5% NFDM/TBST.

    • ab91605 at 1/100 dilution, staining Cardiac Troponin T in paraffin embedded Human cardiac muscle tissue

    Anti-Cardiac Troponin T antibody [EPR3695] (ab91605)参考文献

    This product has been referenced in:
    • Zhang L  et al. Derivation and high engraftment of patient-specific cardiomyocyte sheet using induced pluripotent stem cells generated from adult cardiac fibroblast. Circ Heart Fail 8:156-66 (2015). Human . Read more (PubMed: 25420485) »
    • Moyes KW  et al. Human Embryonic Stem Cell-Derived Cardiomyocytes Migrate in Response to Gradients of Fibronectin and Wnt5a. Stem Cells Dev N/A:N/A (2013). Read more (PubMed: 23517131) »

    See all 3 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Pig Tissue lysate - whole (Heart)
    Gel Running Conditions Reduced Denaturing (12)
    Loading amount 10 µg
    Specification Heart
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: rt°C
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    提交于 Nov 25 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"