Anti-Cardiac Troponin T抗体(ab45932)


  • 产品名称Anti-Cardiac Troponin T抗体
    参阅全部 Cardiac Troponin T 一抗
  • 描述
    兔多克隆抗体to Cardiac Troponin T
  • 经测试应用适用于: IHC-P, Sandwich ELISA, ICC/IF, WBmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Rabbit, Guinea pig, Cow, Cat, Dog不与反应: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human cardiac Troponin T.

    (Peptide available as ab47005.)

  • 阳性对照
    • WB: Human fetal heart tissue lysate. IHC-P: Human heart tissue.



Our Abpromise guarantee covers the use of ab45932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214) as Capture.
ICC/IF 1/400. Incubate for 1-2 hours.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).


  • 功能Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • 组织特异性Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
  • 疾病相关Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
  • 序列相似性Belongs to the troponin T family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cardiac muscle troponin T antibody
    • Cardiomyopathy dilated 1D (autosomal dominant) antibody
    • Cardiomyopathy hypertrophic 2 antibody
    • CMD1D antibody
    • CMH2 antibody
    • CMPD2 antibody
    • cTnT antibody
    • LVNC6 antibody
    • MGC3889 antibody
    • OTTHUMP00000033864 antibody
    • OTTHUMP00000033865 antibody
    • OTTHUMP00000033866 antibody
    • OTTHUMP00000033867 antibody
    • OTTHUMP00000033870 antibody
    • OTTHUMP00000218095 antibody
    • RCM3 antibody
    • TNNT 2 antibody
    • TNNT2 antibody
    • TNNT2_HUMAN antibody
    • TnTc antibody
    • Troponin T cardiac muscle antibody
    • Troponin T type 2 (cardiac) antibody
    • Troponin T type 2 cardiac antibody
    • Troponin T, cardiac muscle antibody
    • Troponin T2 cardiac antibody
    see all

Anti-Cardiac Troponin T antibody 图像

  • ab45932 staining Cardiac Troponin T in rat cardiac cells by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in 3% paraformaldehyde. Samples were incubated with primary antibody (1/400 in diluent) for 1 hour at room temperature. A Cy3®-conjugated goat anti-rabbit polyclonal IgG (1/300) was used as the secondary antibody.

    See Abreview

  • Anti-Cardiac Troponin T antibody (ab45932) at 1 µg/ml + Heart (Human) Whole Cell Lysate - fetal normal tissue at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

    Exposure time : 30 seconds
  • IHC image of Cardiac Troponin T staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45932, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ab45932 staining cardiac Troponin T in human fetal cardiomyocytes by ICC/IF. The cells were paraformaldehyde fixed, permeabilized in 0.1% (PBS) saponin buffer and blocked in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1/400 (0.1% saponin, 10% goat serum, 0.01% triton) and incubated with sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (L+H), diluted 1/600 was used as secondary.

    See Abreview

  • Standard Curve for Cardiac Troponin T (Analyte: Cardiac Troponin T protein (His tag) (ab86685)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214) at 5ug/ml and Detector Antibody Rabbit polyclonal to cardiac Troponin T (ab45932) at 0.5ug/ml.


Anti-Cardiac Troponin T antibody (ab45932)参考文献

This product has been referenced in:
  • Uzun AU  et al. Ca(2+)-Currents in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes Effects of Two Different Culture Conditions. Front Pharmacol 7:300 (2016). WB . Read more (PubMed: 27672365) »
  • Mitzelfelt KA  et al. The Human 343delT HSPB5 Chaperone Associated with Early-onset Skeletal Myopathy Causes Defects in Protein Solubility. J Biol Chem 291:14939-53 (2016). Read more (PubMed: 27226619) »

See all 9 Publications for this product

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Expiration date: DD MM YYYY
I am very pleased to hear you would like to accept our offer and test ab45932 in Rat. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please...

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Application Immunocytochemistry
Sample Human Cultured Cells (differentiated iPSC)
Specification differentiated iPSC
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Paraformaldehyde

Abcam user community

Verified customer

提交于 Apr 12 2016

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Specification Human pluripotent stem cell derived cardiomyocyte
Permeabilization Yes - saponin
Fixative Paraformaldehyde

Abcam user community

Verified customer

提交于 Nov 19 2014

Thank you for your enquiry. We are not aware of any references for the antibody ab45932 that include western blot data demonstrating recognition of intact and fragmented troponin T. There is a western blot of human heart tissue on the datasheet which s...

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Thank you for contacting us.

The antibody ab45932 recognizes the amino acid sequence of the immunogen, so it should be capable of detecting protein fragments that contain this sequence, using the applications listed on the datasheet: western...

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Application Immunocytochemistry/ Immunofluorescence
Sample Rabbit Cell (RAT cardiac cells)
Specification RAT cardiac cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton x-100 in 3% paraformaldehyde

Tova Volberg

Verified customer

提交于 Jul 17 2012

Thank you for your inquiry.

I am assuming your customer wrote an Abreview for ab45932 and rabbit?

If so, I can confirm that we received the Abreview (today 17.07.2012)and one of my colleagues is working on it.

Since the co...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (neonatal cardiomyocyte)
Specification neonatal cardiomyocyte
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% TritonX-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Mrs. Jamie Soto

Verified customer

提交于 Jun 15 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Kardiomyocyten)
Specification Kardiomyocyten
Fixative CytoPerm/Fix (BD)
Permeabilization Yes - WashPerm (BD)
Blocking step WashPerm (BD) as blocking agent for 45 minute(s) · Concentration: 100% · Temperature: 24°C

Abcam user community

Verified customer

提交于 May 07 2012

Thank you for calling Abcam earlier today.

Please let me know if the protocol tips we discussed prove successful or not.

The antibody is covered under our Abpromise for six months and is guaranteed to work in ICC/IF on human samples...

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