Anti-Cardiac Troponin T抗体[7G7] (ab10223)


  • 产品名称Anti-Cardiac Troponin T抗体[7G7]
    参阅全部 Cardiac Troponin T 一抗
  • 描述
    小鼠单克隆抗体[7G7] to Cardiac Troponin T
  • 经测试应用适用于: ELISA, Sandwich ELISA, Other, IHC-P, WBmore details
  • 种属反应性
    与反应: Cow, Human
    不与反应: Mouse, Rat, Rabbit, Goat, Cat, Dog, Pig, Fish
  • 免疫原

    Full length protein (Human).

  • 表位60-70 aa
  • 常规说明Concentration varies from lot to lot and can be provided on request.

    Does not cross-react with skeletal muscle troponin T, cTnT or TnC.



Our Abpromise guarantee covers the use of ab10223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration. Can be paired for ELISA with Mouse monoclonal [1C11] to Cardiac Troponin T (ab8295) and Mouse monoclonal [9G6] to Cardiac Troponin T (HRP) (ab8296).
AP Use at an assay dependent concentration.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [9G6] to Cardiac Troponin T (HRP) (ab8296). Can be used as Capture antibody.
Other Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 35.4 kDa.


  • 功能Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • 组织特异性Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
  • 疾病相关Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
  • 序列相似性Belongs to the troponin T family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cardiac muscle troponin T antibody
    • Cardiomyopathy dilated 1D (autosomal dominant) antibody
    • Cardiomyopathy hypertrophic 2 antibody
    • CMD1D antibody
    • CMH2 antibody
    • CMPD2 antibody
    • cTnT antibody
    • LVNC6 antibody
    • MGC3889 antibody
    • OTTHUMP00000033864 antibody
    • OTTHUMP00000033865 antibody
    • OTTHUMP00000033866 antibody
    • OTTHUMP00000033867 antibody
    • OTTHUMP00000033870 antibody
    • OTTHUMP00000218095 antibody
    • RCM3 antibody
    • TNNT 2 antibody
    • TNNT2 antibody
    • TNNT2_HUMAN antibody
    • TnTc antibody
    • Troponin T cardiac muscle antibody
    • Troponin T type 2 (cardiac) antibody
    • Troponin T type 2 cardiac antibody
    • Troponin T, cardiac muscle antibody
    • Troponin T2 cardiac antibody
    see all

Anti-Cardiac Troponin T antibody [7G7] 图像

  • Ab10223 staining human normal or malignant tissue type. Staining is localised to the cytoplasm .
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Anti-Cardiac Troponin T antibody [7G7] (ab10223) at 1/2000 dilution + Cow heart extract at 20 µg

    HRP-conjugated goat anti-mouse polyclonal at 1/2000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35.4 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

    Exposure time : 10 seconds

    This image is courtesy of an Anonymous Abreview.

    See Abreview

Anti-Cardiac Troponin T antibody [7G7] (ab10223)参考文献

ab10223 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Cow Tissue lysate - whole (Heart)
Loading amount 20 µg
Specification Heart
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

Abcam user community

Verified customer

提交于 Jul 21 2011