Anti-Cardiac Troponin T抗体[1F11] (ab10214)

概述

  • 产品名称Anti-Cardiac Troponin T抗体[1F11]
    参阅全部 Cardiac Troponin T 一抗
  • 描述
    小鼠单克隆抗体[1F11] to Cardiac Troponin T
  • 特异性Suitable for use in Surface Plasmon Resonance Biosensing (PubMed: 20633583 and 20694813)
  • 经测试应用适用于: Flow Cyt, ELISA, WB, Sandwich ELISA, ICC, Other, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Rabbit, Goat, Cow, Cat, Dog, Human, Pig, Fish
  • 免疫原

    Free human cardiac troponin T

  • 表位Peptide 146-160.
  • 常规说明Concentration varies from lot to lot and can be provided on request.


    Does not cross-react with skeletal muscle troponin T, cTnI or TnC.

性能

应用

Our Abpromise guarantee covers the use of ab10214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. PubMed: 21278734

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

AP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 35.4 kDa.
Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Cardiac Troponin T (ab45932). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to cardiac Troponin T (ab45932) as Detection.
ICC Use at an assay dependent concentration. Used at a dilution of 1/400 for 1 hr incubation on rat cardiomyocytes (see Abreview for further details).
Other Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 23066088

靶标

  • 功能Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • 组织特异性Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
  • 疾病相关Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
  • 序列相似性Belongs to the troponin T family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cardiac muscle troponin T antibody
    • Cardiomyopathy dilated 1D (autosomal dominant) antibody
    • Cardiomyopathy hypertrophic 2 antibody
    • CMD1D antibody
    • CMH2 antibody
    • CMPD2 antibody
    • cTnT antibody
    • LVNC6 antibody
    • MGC3889 antibody
    • OTTHUMP00000033864 antibody
    • OTTHUMP00000033865 antibody
    • OTTHUMP00000033866 antibody
    • OTTHUMP00000033867 antibody
    • OTTHUMP00000033870 antibody
    • OTTHUMP00000218095 antibody
    • RCM3 antibody
    • TNNT 2 antibody
    • TNNT2 antibody
    • TNNT2_HUMAN antibody
    • TnTc antibody
    • Troponin T cardiac muscle antibody
    • Troponin T type 2 (cardiac) antibody
    • Troponin T type 2 cardiac antibody
    • Troponin T, cardiac muscle antibody
    • Troponin T2 cardiac antibody
    see all

Anti-Cardiac Troponin T antibody [1F11] 图像

  • Standard Curve for Cardiac Troponin T (Analyte: Cardiac Troponin T protein (His tag) (ab86685)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214) at 5ug/ml and Detector Antibody Rabbit polyclonal to cardiac Troponin T (ab45932) at 0.5ug/ml.
  • ab10214 staining human spontaneous differentiating iPSC by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with PFA, permeabilized with 0.25%% Triton X-100 in PBS and blocked with 1% BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 16 hours at 4°C. An undiluted Alexa Fluor® 594-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-Cardiac Troponin T antibody [1F11] (ab10214) at 1/2000 dilution

    Lane 1 : Mouse heart whole tissue lysate
    Lane 2 : Mouse heart whole tissue lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    An HRP-conjugated Goat anti-mouse polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 35.4 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an anonymous Abreview

    Blocking Step: 5% Milk for 1 hour at room temperature.

    See Abreview

  • ab10214 at a 1/400 dilution staining rat cardiomyocytes by immunocytochemistry. The cells were paraformaldehyde fixed and blocked with 2% serum. The antibody was incubated with the cells for 16 hours and then bound antibody was detected using an Alexa Fluor® 594 conjugated donkey Anti-Mouse IgG(H+L).

    This image is courtesy of an Abreview submitted by Yiqiang Zhang.

    See Abreview

实验方案

Anti-Cardiac Troponin T antibody [1F11] (ab10214)参考文献

This product has been referenced in:
  • Wang Y  et al. The transplantation of Akt-overexpressing amniotic fluid-derived mesenchymal stem cells protects the heart against ischemia-reperfusion injury in rabbits. Mol Med Rep 14:234-42 (2016). IHC-P ; Rabbit . Read more (PubMed: 27151366) »
  • Jackson R  et al. Paracrine Engineering of Human Cardiac Stem Cells With Insulin-Like Growth Factor 1 Enhances Myocardial Repair. J Am Heart Assoc 4:e002104 (2015). Read more (PubMed: 26363004) »

See all 11 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (spontaneous differentiating iPSC)
Permeabilization Yes - 0.25% Triton X-100 in PBS
Specification spontaneous differentiating iPSC
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 10 2016

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (Cardiac cells)
Total protein in input 50 µg
Immuno-precipitation step Protein A/G
Specification Cardiac cells
Username

Abcam user community

Verified customer

提交于 Jun 27 2015

Thank you for your contact. I am very sorry to hear that the vial was empty.
This is to let you know that I have placed a new order as a free of charge replacement of ab10214. The new order number is 1217998.
If you need any further assistanc...

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Thank you for contacting us.
I am sorry to hear that the antibody that worked previously is not working anymore.

I checked our records for this lot number, but have not found any complaints. We do not test any antibodies in SPR, thus in ge...

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Thank you for contacting us and for providing this image.

After review of the image provided, we believe that the extra bands which you are seeing are result of non-specific background. This will show up when using too much protein, too mu...

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I could certainly offer you a code number. Would you be so kind to confirm the name and the address of your research institute? We need this information only for our internal record.

Thank you and I look forward to hearing from you ...

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Thank you for your enquiry and your interest in our products.

I can confirm that these three antibodies recognize the mouse protein and the host species in which they were raised is also mouse. This may cause some non-specificity signal probl...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Cow Tissue lysate - whole (heart tissue)
Loading amount 20 µg
Specification heart tissue
Gel Running Conditions Reduced Denaturing (13)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Feb 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (ES cell EBs differentiated for 12 days.)
Specification ES cell EBs differentiated for 12 days.
Fixative Methanol
Permeabilization Yes - 0.1%triton-x
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Apr 22 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Rat Cell (cardiomyocytes)
Specification cardiomyocytes
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2
Username

Dr. Yiqiang Zhang

Verified customer

提交于 Jun 09 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"