Anti-Cardiac Troponin T抗体[13-11], prediluted (ab74275)

概述

  • 产品名称
    Anti-Cardiac Troponin T抗体[13-11], prediluted
    参阅全部 Cardiac Troponin T 一抗
  • 描述
    小鼠单克隆抗体[13-11] to Cardiac Troponin T, prediluted
  • 特异性
    We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用
    适用于: IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Chicken, Guinea pig, Dog, Human, Pig
  • 免疫原

    Purified rabbit cardiac troponin T isoform (TnT4R)

  • 表位
    FMPNLVPPKI (5’ region of the cardiac gene exon 10).
  • 阳性对照
    • Cardiac muscle.
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

性能

应用

Our Abpromise guarantee covers the use of ab74275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
Flow Cyt
  • 应用说明
    Flow Cyt: Use at an assay dependent dilution (PMID 19910576).
    IHC-P: Ready-to-use. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
    • 组织特异性
      Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
    • 疾病相关
      Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
      Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
      Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
    • 序列相似性
      Belongs to the troponin T family.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cardiac muscle troponin T antibody
      • Cardiomyopathy dilated 1D (autosomal dominant) antibody
      • Cardiomyopathy hypertrophic 2 antibody
      • CMD1D antibody
      • CMH2 antibody
      • CMPD2 antibody
      • cTnT antibody
      • LVNC6 antibody
      • MGC3889 antibody
      • OTTHUMP00000033864 antibody
      • OTTHUMP00000033865 antibody
      • OTTHUMP00000033866 antibody
      • OTTHUMP00000033867 antibody
      • OTTHUMP00000033870 antibody
      • OTTHUMP00000218095 antibody
      • RCM3 antibody
      • TNNT 2 antibody
      • TNNT2 antibody
      • TNNT2_HUMAN antibody
      • TnTc antibody
      • Troponin T cardiac muscle antibody
      • Troponin T type 2 (cardiac) antibody
      • Troponin T type 2 cardiac antibody
      • Troponin T, cardiac muscle antibody
      • Troponin T2 cardiac antibody
      see all

    图片

    • Formalin-fixed, paraffin-embedded rat heart stained with ab74275 using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of cardiac muscles.
    • Formalin-fixed, paraffin-embedded human heart stained with ab74275 using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of cardiac muscle cells.

    文献

    This product has been referenced in:
    • Sharifi-Sanjani M  et al. Cardiomyocyte-Specific Telomere Shortening is a Distinct Signature of Heart Failure in Humans. J Am Heart Assoc 6:N/A (2017). IHC-P ; Human . Read more (PubMed: 28882819) »
    • Chang AC  et al. Telomere shortening and metabolic compromise underlie dystrophic cardiomyopathy. Proc Natl Acad Sci U S A 113:13120-13125 (2016). IF ; Mouse . Read more (PubMed: 27799523) »

    See all 4 Publications for this product

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