Anti-cardiac Troponin I (phospho S43)抗体(ab59420)

概述

  • 产品名称Anti-cardiac Troponin I (phospho S43)抗体
    参阅全部 Cardiac Troponin I 一抗
  • 描述
    兔多克隆抗体to cardiac Troponin I (phospho S43)
  • 特异性Detects endogenous levels of Troponin I only when phosphorylated at serine 43
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from human cardiac troponin I around the phosphorylation site of serine 43 (S-A-SP-R-K).

  • 阳性对照
    • Jurkat Cells

性能

应用

Our Abpromise guarantee covers the use of ab59420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 24 kDa).
ELISA 1/40000.

靶标

  • 功能Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • 疾病相关Defects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • 序列相似性Belongs to the troponin I family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • cardiac muscle antibody
    • Cardiac troponin I antibody
    • cardiomyopathy, dilated 2A (autosomal recessive) antibody
    • Cardiomyopathy, familial hypertrophic, 7, included antibody
    • CMD1FF antibody
    • CMD2A antibody
    • CMH7 antibody
    • cTnI antibody
    • Familial hypertrophic cardiomyopathy 7 antibody
    • MGC116817 antibody
    • RCM1 antibody
    • Tn1 antibody
    • Tni antibody
    • TNN I3 antibody
    • TNNC 1 antibody
    • TNNC1 antibody
    • TNNI3 antibody
    • TNNI3_HUMAN antibody
    • Troponin I antibody
    • Troponin I cardiac antibody
    • Troponin I cardiac muscle antibody
    • Troponin I cardiac muscle isoform antibody
    • Troponin I type 3 cardiac antibody
    • troponin I, cardiac 3 antibody
    • TroponinI antibody
    • Ttroponin I type 3 (cardiac) antibody
    see all

Anti-cardiac Troponin I (phospho S43) antibody 图像

  • All lanes : Anti-cardiac Troponin I (phospho S43) antibody (ab59420) at 1/500 dilution

    Lane 1 : Jurkat cells
    Lane 2 : Jurkat cells with blocking phosphopeptide


    Predicted band size : 24 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

Anti-cardiac Troponin I (phospho S43) antibody (ab59420)参考文献

This product has been referenced in:
  • Yu ZY  et al. RhoA/ROCK Signaling and Pleiotropic a1A-Adrenergic Receptor Regulation of Cardiac Contractility. PLoS One 9:e99024 (2014). WB ; Mouse . Read more (PubMed: 24919197) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Recombinant protein (Cardiac)
Loading amount 3 µg
Specification Cardiac
Treatment PKA and/or PKC treated
Gel Running Conditions Reduced Denaturing (12% discontinuous SDS-PAGE, PVDF membrane)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Verified customer

提交于 Jul 30 2008

Application Western blot
Sample Mouse Cell lysate - whole cell (Cardiac myocytes)
Loading amount 15000 cells
Specification Cardiac myocytes
Treatment 20uM phenylephrine
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 May 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"