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free human cardiac troponin and/or native troponin complex.
Our Abpromise guarantee covers the use of ab10239 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Other||Use at an assay dependent concentration.|
|Sandwich ELISA||Use at an assay dependent concentration. Can be used as Detection ab. The ELISA based on recommended Abs, recognizes complexed and free forms with same sensitivity. For better sensitivity and reproducibility we recommend using at least one ab that recognizes the stable fragment of cTnI (30-110 aa), as cTnI is proteolytically degraded in the bloodstream. Because the amount of complexed cTnI in blood of AMI patients is very high, we recommend using a MAb specific to TnC or a MAb specific to TnC together with MAb(s) specific to cTnI.|
IHC image of Cardiac Troponin I staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10239, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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