Anti-Cardiac FABP抗体(ab45966)

概述

  • 产品名称
    Anti-Cardiac FABP抗体
    参阅全部 Cardiac FABP 一抗
  • 描述
    兔多克隆抗体to Cardiac FABP
  • 特异性
    ab45966 reacts with cardiac FABP, but does not react with liver or intestinal forms of this protein.
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Cardiac FABP.

    (Peptide available as ab46185.)

  • 阳性对照
    • ab45966 gives a positive result in the following tissue lysates: Heart (Human) Tissue Lysate, Skeletal Muscle (Human) Tissue Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab45966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
IHC-P 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能
    FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.
  • 序列相似性
    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • 结构域
    Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 422 protein antibody
    • Cardiac Fatty Acid Binding Protein antibody
    • FABP 11 antibody
    • FABP 3 antibody
    • FABP11 antibody
    • FABP3 antibody
    • FABPH_HUMAN antibody
    • Fatty acid binding protein 11 antibody
    • Fatty acid binding protein 3 antibody
    • Fatty acid binding protein 3 muscle and heart antibody
    • Fatty acid binding protein 3 muscle and heart mammary derived growth inhibitor antibody
    • Fatty acid binding protein 3 muscle antibody
    • Fatty acid binding protein 3, muscle and heart (mammary derived growth inhibitor) antibody
    • Fatty acid binding protein 3, muscle antibody
    • Fatty acid binding protein heart antibody
    • Fatty acid binding protein, heart antibody
    • Fatty acid binding protein, muscle and heart antibody
    • Fatty acid binding protein, skeletal muscle antibody
    • Fatty acid-binding protein 3 antibody
    • Fatty acid-binding protein antibody
    • H FABP antibody
    • H-FABP antibody
    • heart antibody
    • Heart type fatty acid binding protein antibody
    • Heart-type fatty acid-binding protein antibody
    • M FABP antibody
    • M-FABP antibody
    • Mammary derived growth inhibitor antibody
    • Mammary-derived growth inhibitor antibody
    • MDGI antibody
    • Muscle fatty acid binding protein antibody
    • Muscle fatty acid-binding protein antibody
    • Mylein protein P2 homolog antibody
    • O FABP antibody
    • OTTHUMP00000003898 antibody
    • P2 adopocyte protein antibody
    see all

Anti-Cardiac FABP antibody 图像

  • All lanes : Anti-Cardiac FABP antibody (ab45966) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human heart tissue lysate - total protein (ab29431)
    Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 4 : Human small intestine tissue lysate - total protein (ab29276)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 15 kDa
    Observed band size : 15 kDa
  • ab45966, at a 1/500 dilution, staining cardiac FABP in mouse heart by Immunohistochemistry, using formalin-fixed paraffin embedded tissue.

    See Abreview

  • Anti-Cardiac FABP antibody (ab45966) at 1 µg/ml + Recombinant Human Cardiac FABP protein (ab109160) at 1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 8 minutes

Anti-Cardiac FABP antibody (ab45966)参考文献

This product has been referenced in:
  • Antohe F  et al. Heart microvessels and aortic endothelial cells express the 15 kDa heart-type fatty acid-binding proteins. Eur J Cell Biol 76:102-9 (1998). IHC-P ; Rat . Read more (PubMed: 9696349) »

See 1 Publication for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Rat Placenta)
Antigen retrieval step
Enzymatic
Permeabilization
No
Specification
Rat Placenta
Blocking step
Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1.5%
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 26 2016

Thank you for contacting us and reporting the difficulties you have been experiencing in using the anti-Cardiac FABP antibody (ab45966).

I am sorry to hear that this antibody has not been proving sensitive enough for you currently. There are ...

Read More

DISCOUNT CODE: *******
Expiration date*******

I am very pleased to hear you would like to accept our offer and test ab28723 in Zebrafinches. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offe...

Read More

Thank you for your inquiry. We do not assign strict expiration dates for our products, however we follow general guidelines: Antibodies stored at 4C may be viable up to 1 year, those stored at -20C may be viable for up to 5 years, and those stored at -...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human cardiomyocytes)
Loading amount
20 µg
Specification
Human cardiomyocytes
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE gel with a 5% stacking gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Prof. Paul Townsend

Verified customer

提交于 Mar 18 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (HEART, INTESTINE)
Loading amount
20 µg
Specification
HEART, INTESTINE
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Mar 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (heart)
Specification
heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Username

Abcam user community

Verified customer

提交于 Feb 11 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (heart)
Specification
heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Aug 20 2008

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (heart)
Specification
heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Aug 20 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (heart)
Specification
heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH6.0
Permeabilization
No
Blocking step
Sequential peroxidase (5min) and protein (10min) block (prediluted) as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Aug 19 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册