Anti-H-FABP抗体(ab45966)
Key features and details
- Rabbit polyclonal to H-FABP
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-H-FABP抗体
参阅全部 H-FABP 一抗 -
描述
兔多克隆抗体to H-FABP -
宿主
Rabbit -
特异性
ab45966 reacts with H-FABP, but does not react with liver or intestinal forms of this protein.
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Human, Recombinant fragment
预测可用于: Mouse, Rat, Cow, Pig -
免疫原
Synthetic peptide corresponding to Human H-FABP aa 100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab46185) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab45966于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa). |
靶标
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功能
FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. -
序列相似性
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
结构域
Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 281758 Cow
- Entrez Gene: 2170 Human
- Entrez Gene: 14077 Mouse
- Entrez Gene: 399532 Pig
- Entrez Gene: 79131 Rat
- Omim: 134651 Human
- SwissProt: P10790 Cow
- SwissProt: P05413 Human
see all -
别名
- 422 protein antibody
- Cardiac FABP antibody
- Cardiac Fatty Acid Binding Protein antibody
see all
图片
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All lanes : Anti-H-FABP antibody (ab45966) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 4 : Human small intestine tissue lysate - total protein (ab29276)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Ab45966 does not cross-react with small intestine or liver FABP. -
Ab45966 recognizes the full length tagged recombinant protein (ab109160) which has an expected molecular weight of 16 kDa.
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab45966 被引用在 12 文献中.
- Liu ZZ et al. Autophagy receptor OPTN (optineurin) regulates mesenchymal stem cell fate and bone-fat balance during aging by clearing FABP3. Autophagy 17:2766-2782 (2021). PubMed: 33143524
- Chassen SS et al. Placental fatty acid transport across late gestation in a baboon model of intrauterine growth restriction. J Physiol 598:2469-2489 (2020). PubMed: 32338384
- Wang X et al. Inhibition of miR-27b Regulates Lipid Metabolism in Skeletal Muscle of Obese Rats During Hypoxic Exercise by Increasing PPAR? Expression. Front Physiol 11:1090 (2020). PubMed: 32982800
- Baati N et al. New evidence of exercise training benefits in myostatin-deficient mice: Effect on lipidomic abnormalities. Biochem Biophys Res Commun 516:89-95 (2019). PubMed: 31200956
- Shrestha N et al. Linoleic Acid Increases Prostaglandin E2 Release and Reduces Mitochondrial Respiration and Cell Viability in Human Trophoblast-Like Cells. Cell Physiol Biochem 52:94-108 (2019). PubMed: 30790507