Anti-H-FABP抗体[67D3] (ab16916)
Key features and details
- Mouse monoclonal [67D3] to H-FABP
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-H-FABP抗体[67D3]
参阅全部 H-FABP 一抗 -
描述
小鼠单克隆抗体[67D3] to H-FABP -
宿主
Mouse -
特异性
Ab16916 binds to human H-FABP. It shows no binding to human intestinal or liver FABP and slightly cross reacts with B-FABP.
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经测试应用
适用于: IHC-P, IHC-Frmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Full length native protein (purified) corresponding to Human H-FABP.
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阳性对照
- Heart cells, recombinant human H-FABP This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.
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常规说明
This antibody can be used to detect ischemia areas of human heart. It is also useful as marker for brain damage.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Provided as a 0.2µm filtered antibody solution. -
Primary antibody说明
This antibody can be used to detect ischemia areas of human heart. It is also useful as marker for brain damage. -
克隆
单克隆 -
克隆编号
67D3 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab16916于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (1) |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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说明 |
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IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
靶标
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功能
FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. -
序列相似性
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
结构域
Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 2170 Human
- Entrez Gene: 14077 Mouse
- Entrez Gene: 79131 Rat
- Omim: 134651 Human
- SwissProt: P05413 Human
- SwissProt: P11404 Mouse
- SwissProt: P07483 Rat
- Unigene: 657242 Human
see all -
别名
- 422 protein antibody
- Cardiac FABP antibody
- Cardiac Fatty Acid Binding Protein antibody
see all
图片
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Immunohistochemical staining of H-FABP in human skeletal muscle tissue.
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IHC image of H-FABP staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16916, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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Datasheet download
文献 (6)
ab16916 被引用在 6 文献中.
- Belcastro L et al. Decreased Fatty Acid Transporter FABP1 and Increased Isoprostanes and Neuroprostanes in the Human Term Placenta: Implications for Inflammation and Birth Weight in Maternal Pre-Gestational Obesity. Nutrients 13:N/A (2021). PubMed: 34444927
- Khan D et al. SIRT6 transcriptionally regulates fatty acid transport by suppressing PPARγ. Cell Rep 35:109190 (2021). PubMed: 34077730
- Jones HB et al. Myocardial Steatosis and Necrosis in Atria and Ventricles of Rats Given Pyruvate Dehydrogenase Kinase Inhibitors. Toxicol Pathol N/A:N/A (2014). PubMed: 24742628
- Chen HM et al. Heart-type Fatty Acid binding protein is associated with proteinuria in obesity. PLoS One 7:e45691 (2012). IHC-P, IHC-Fr ; Mouse . PubMed: 23029183
- Heather LC et al. Adenosine monophosphate-activated protein kinase activation, substrate transporter translocation, and metabolism in the contracting hyperthyroid rat heart. Endocrinology 151:422-31 (2010). WB ; Rat . PubMed: 19940039
- Nevo J et al. Mammary-derived growth inhibitor alters traffic of EGFR and induces a novel form of cetuximab resistance. Clin Cancer Res 15:6570-81 (2009). IHC-P ; Human . PubMed: 19825952