RabMAb

Anti-CARD9抗体[EPR6488] (ab124922)

概述

  • 产品名称Anti-CARD9抗体[EPR6488]
    参阅全部 CARD9 一抗
  • 描述
    兔单克隆抗体[EPR6488] to CARD9
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • 阳性对照
    • THP1, Raji, Jurkat, and PC3 cell lysates; Human brain and liver tissues
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 解离常数(KD KD = 1.79 x 10 -10 M Learn more about KD
  • 存储溶液pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号EPR6488
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab124922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for Flow Cyt,ICC or IP.
  • 靶标

    • 功能Activates NF-kappa-B via BCL10.
    • 组织特异性Highly expressed in spleen. Also detected in liver, placenta, lung, peripheral blood leukocytes and in brain.
    • 疾病相关Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2) [MIM:212050]. Chronic mucocutaneous candidiasis is characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CANDF2 is an autosomal recessive chronic mucocutaneous candidiasis.
    • 序列相似性Contains 1 CARD domain.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CANDF2 antibody
      • CARD9 antibody
      • CARD9_HUMAN antibody
      • Caspase recruitment domain family member 9 antibody
      • Caspase recruitment domain-containing protein 9 antibody
      • hCARD9 antibody
      see all

    Anti-CARD9 antibody [EPR6488] 图像

    • All lanes : Anti-CARD9 antibody [EPR6488] (ab124922) at 1/1000 dilution

      Lane 1 : THP1 cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : PC3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 62 kDa
      Observed band size : 62 kDa
    • ab124922, at 1/250 dilution, staining CARD9 in paraffin-embedded Human brain tissue by Immunohistochemistry.
    • ab124922, at 1/250 dilution, staining CARD9 in paraffin-embedded Human liver tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-CARD9 antibody [EPR6488] (ab124922)参考文献

    ab124922 has not yet been referenced specifically in any publications.

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