Anti-CARD8抗体(ab24186)
Key features and details
- Rabbit polyclonal to CARD8
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CARD8抗体
参阅全部 CARD8 一抗 -
描述
兔多克隆抗体to CARD8 -
宿主
Rabbit -
特异性
ab24186 reacts with CARD8 (CARDINAL/TUCAN) protein. -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to amino acids within the last 50 amino acids of the C terminus of human CARD8, GenBank accession AAG50014
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab24186于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF | (1) |
Use a concentration of 10 µg/ml.
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WB | (1) |
Use a concentration of 2 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa).
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说明 |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa). |
靶标
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功能
Inhibits NF-kappa-B activation. May participate in a regulatory mechanism that coordinates cellular responses controlled by NF-kappa-B transcription factor. May be a component of the inflammasome, a protein complex which also includes PYCARD, NALP2 and CASP1 and whose function would be the activation of proinflammatory caspases. -
组织特异性
High expression in lung, ovary, testis and placenta. Lower expression in heart, kidney and liver. Also expressed in spleen, lymph node and bone marrow. -
序列相似性
Contains 1 CARD domain. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 22900 Human
- Omim: 609051 Human
- SwissProt: Q9Y2G2 Human
- Unigene: 446146 Human
- Unigene: 655940 Human
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别名
- Apoptotic protein NDPP 1 antibody
- Apoptotic protein NDPP1 antibody
- CARD 8 antibody
see all
图片
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Lane 1 : Anti-CARD8 antibody (ab24186) at 2 µg/ml (CARD8 expression in K562 cell lysate)
Lane 2 : Anti-CARD8 antibody (ab24186) at 4 µg/ml (CARD8 expression in K562 cell lysate)
All lanes :
Predicted band size: 49 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
A peptide blocking study was also done (data not shown) and the only bands that disappeared were around 45kDa. The other bands are thought to be non-specific, but more testing to confirm or refute this has not been performed. -
ab24186 at 10µg/ml staining CARD8 in K562 cells by ICC/IF
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ab24186 staining CARD8 in human monocytes by Immunocytochemistry/ Immunofluorescence.
Cells were fixed, permeabilized, blocked with 2% BSA for 30 minutes at 25°C and then incubated with ab24186 at a 1/200 dilution for 4 hours at 25°C. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) used at a 1/1000 dilution. -
ab24186 staining CARD8 in Mouse RAW 264.7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 in 2% BSA and blocked with 2% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/200 in PBS + 2% BSA) for 18 hours at 4°C. An Alexa Fluor®488-conjugated Chicken anti-rabbit IgG polyclonal (1/750) was used as the secondary antibody.
WGA = Wheat Germ Agglutinin
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab24186 被引用在 10 文献中.
- Hsiao JC et al. A ubiquitin-independent proteasome pathway controls activation of the CARD8 inflammasome. J Biol Chem 298:102032 (2022). PubMed: 35580636
- Sharif H et al. Dipeptidyl peptidase 9 sets a threshold for CARD8 inflammasome formation by sequestering its active C-terminal fragment. Immunity 54:1392-1404.e10 (2021). PubMed: 34019797
- Ball DP et al. Caspase-1 interdomain linker cleavage is required for pyroptosis. Life Sci Alliance 3:N/A (2020). PubMed: 32051255
- Chui AJ et al. Activation of the CARD8 Inflammasome Requires a Disordered Region. Cell Rep 33:108264 (2020). PubMed: 33053349
- Linder A et al. CARD8 inflammasome activation triggers pyroptosis in human T cells. EMBO J 39:e105071 (2020). PubMed: 32840892
- Johnson DC et al. DPP8/9 inhibitors activate the CARD8 inflammasome in resting lymphocytes. Cell Death Dis 11:628 (2020). PubMed: 32796818
- Johnson DC et al. DPP8/DPP9 inhibitor-induced pyroptosis for treatment of acute myeloid leukemia. Nat Med 24:1151-1156 (2018). PubMed: 29967349
- D'Osualdo A et al. CARD8 and NLRP1 undergo autoproteolytic processing through a ZU5-like domain. PLoS One 6:e27396 (2011). WB ; Human . PubMed: 22087307
- Mille F et al. The Patched dependence receptor triggers apoptosis through a DRAL-caspase-9 complex. Nat Cell Biol 11:739-46 (2009). WB ; Human . PubMed: 19465923
- Ko DC et al. A genome-wide in vitro bacterial-infection screen reveals human variation in the host response associated with inflammatory disease. Am J Hum Genet 85:214-27 (2009). WB ; Human . PubMed: 19664744