Anti-CARD8抗体[2108C2a] (ab50977)

概述

  • 产品名称Anti-CARD8抗体[2108C2a]
    参阅全部 CARD8 一抗
  • 描述
    小鼠单克隆抗体[2108C2a] to CARD8
  • 经测试应用适用于: WB, IP, Dot blotmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant human CARD8 fragment

  • 表位ab50977 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
  • 阳性对照
    • HeLa whole cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, 8.0mM Sodium phosphate, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号2108C2a
  • 同种型IgG1
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab50977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
Dot blot
  • 应用说明Dot: Use at an assay dependent dilution.
    IP: Use 5µg/200ul HeLa RIPA lysate.
    WB: Use at an assay dependent dilution. Detects a band of approximately 46 kDa (predicted molecular weight: 49 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Inhibits NF-kappa-B activation. May participate in a regulatory mechanism that coordinates cellular responses controlled by NF-kappa-B transcription factor. May be a component of the inflammasome, a protein complex which also includes PYCARD, NALP2 and CASP1 and whose function would be the activation of proinflammatory caspases.
    • 组织特异性High expression in lung, ovary, testis and placenta. Lower expression in heart, kidney and liver. Also expressed in spleen, lymph node and bone marrow.
    • 序列相似性Contains 1 CARD domain.
    • 细胞定位Cytoplasm. Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Apoptotic protein NDPP 1 antibody
      • Apoptotic protein NDPP1 antibody
      • CARD 8 antibody
      • CARD inhibitor of NF kappa B activating ligand antibody
      • CARD inhibitor of NF kappaB activating ligand antibody
      • CARD inhibitor of NF kappaB activating ligands antibody
      • CARD-inhibitor of NF-kappa-B-activating ligand antibody
      • CARD8 antibody
      • CARD8_HUMAN antibody
      • CARDINAL antibody
      • Caspase recruitment domain containing protein 8 antibody
      • Caspase recruitment domain family member 8 antibody
      • Caspase recruitment domain protein 8 antibody
      • Caspase recruitment domain-containing protein 8 antibody
      • DACAR antibody
      • Dakar antibody
      • DKFZp779L0366 antibody
      • FLJ18119 antibody
      • FLJ18121 antibody
      • KIAA0955 antibody
      • MGC57162 antibody
      • NDPP 1 antibody
      • NDPP antibody
      • NDPP1 antibody
      • TUCAN antibody
      • Tumor up regulated CARD containing antagonist of CASP9 antibody
      • Tumor up regulated CARD containing antagonist of caspase 9 antibody
      • Tumor up-regulated CARD-containing antagonist of CASP9 antibody
      • tumor up-regulated CARD-containing antagonist of caspase nine antibody
      • Tumor upregulated CARD containing antagonist of CASP9 antibody
      see all

    Anti-CARD8 antibody [2108C2a] 图像

    • Anti-CARD8 antibody [2108C2a] (ab50977) at 1/200 dilution + HeLa whole cell lysate at 25 µg

      Secondary
      anti-mouse IgG at 1/2500 dilution
      Developed using the ECL technique

      Predicted band size : 49 kDa
      Observed band size : 46 kDa (why is the actual band size different from the predicted?)
    • Immunoprecipitaion analysis uisng ab50977 at 5ug per 200ul HeLa cell RIPA lysate. Immunoblot: ab50977 at 1/200 dilution using 1/1000 anti-mouse IgG as a secondary antibody.

    Anti-CARD8 antibody [2108C2a] (ab50977)参考文献

    ab50977 has not yet been referenced specifically in any publications.

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