The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明Dot: Use at an assay dependent dilution.
IP: Use 5µg/200ul HeLa RIPA lysate.
WB: Use at an assay dependent dilution. Detects a band of approximately 46 kDa (predicted molecular weight: 49 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Inhibits NF-kappa-B activation. May participate in a regulatory mechanism that coordinates cellular responses controlled by NF-kappa-B transcription factor. May be a component of the inflammasome, a protein complex which also includes PYCARD, NALP2 and CASP1 and whose function would be the activation of proinflammatory caspases.
组织特异性High expression in lung, ovary, testis and placenta. Lower expression in heart, kidney and liver. Also expressed in spleen, lymph node and bone marrow.