概述

  • 产品名称
  • 描述
    兔多克隆抗体to CARD4
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Pig
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 698-928 of Human CARD4 (NP_006083).

  • 阳性对照
    • 293T, A431, H1299, HeLa, HepG2 and Raji cell lysates for WB; HeLa cells for IF.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab97278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 108 kDa.
ICC/IF 1/100 - 1/200.

靶标

  • 功能
    Enhances caspase-9-mediated apoptosis. Induces NF-kappa-B activity via RIPK2 and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Forms an intracellular sensing system along with ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIPK2 dependent NF-kappa-B signaling pathway activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides but also in the activation of NF-kappa-B by Shigella effector proteins IpgB2 and OspB. Recruits NLRP10 to the cell membrane following bacterial infection.
  • 组织特异性
    Highly expressed in adult heart, skeletal muscle, pancreas, spleen and ovary. Also detected in placenta, lung, liver, kidney, thymus, testis, small intestine and colon.
  • 序列相似性
    Contains 1 CARD domain.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • 翻译后修饰
    Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF34 promotes proteasomal degradation and thereby negatively regulates NOD1 for instance in NF-kappa-B activition.
  • 细胞定位
    Cytoplasm. Cell membrane. Apical cell membrane. Basolateral cell membrane. Detected in the cytoplasm and at the cell membrane. Following bacterial infection, localizes to bacterial entry sites in the cell membrane. Recruited to the basolateral and apical membranes in polarized epithelial cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CARD 4 antibody
    • CARD4 antibody
    • Caspase recruitment domain 4 antibody
    • Caspase recruitment domain containing protein 4 antibody
    • Caspase recruitment domain family member 4 antibody
    • Caspase recruitment domain protein 4 antibody
    • Caspase recruitment domain-containing protein 4 antibody
    • CLR 7.1 antibody
    • CLR7.1 antibody
    • NLR family CARD domain containing 1 antibody
    • NLRC 1 antibody
    • NLRC1 antibody
    • NOD 1 antibody
    • Nod1 antibody
    • NOD1 protein antibody
    • NOD1_HUMAN antibody
    • Nucleotide binding oligomerization domain containing 1 antibody
    • Nucleotide binding oligomerization domain leucine rich repeat and CARD domain containing 1 antibody
    • Nucleotide-binding oligomerization domain-containing protein 1 antibody
    • Protein Nod1 antibody
    see all

图片

  • Anti-CARD4 antibody (ab97278) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size : 108 kDa
    7.5% SDS PAGE
  • ab97278 at 1/200 dilution staining CARD4 in paraformaldehyde-fixed HeLa cells by immunofluorescence. The lower image shows ab97278 merged with a DNA probe.

文献

This product has been referenced in:
  • Qian YJ  et al. Cigarette Smoke Modulates NOD1 Signal Pathway and Human ß Defensins Expression in Human Oral Mucosa. Cell Physiol Biochem 36:457-73 (2015). Human . Read more (PubMed: 25968832) »

See 1 Publication for this product

客户评价及客户问答

There are currently no Customer reviews or Questions for ab97278.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册