概述

  • 产品名称Anti-CARD4抗体
    参阅全部 CARD4 一抗
  • 描述
    兔多克隆抗体to CARD4
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human, Monkey
  • 免疫原

    Recombinant fusion protein:

    CLNGNLIKPEEAKVYEDEKR

    , corresponding to C terminal amino acids 930-949 of CARD4.

  • 阳性对照
    • Recombinant fusion protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.025% Sodium Azide
    Constituents: 50% Glycerol, Whole serum
  • 纯度Whole antiserum
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab22143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2500. Predicted molecular weight: 106 kDa.
IHC-P Use at an assay dependent concentration. PubMed: 20844241

靶标

  • 功能Enhances caspase-9-mediated apoptosis. Induces NF-kappa-B activity via RIPK2 and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Forms an intracellular sensing system along with ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIPK2 dependent NF-kappa-B signaling pathway activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides but also in the activation of NF-kappa-B by Shigella effector proteins IpgB2 and OspB. Recruits NLRP10 to the cell membrane following bacterial infection.
  • 组织特异性Highly expressed in adult heart, skeletal muscle, pancreas, spleen and ovary. Also detected in placenta, lung, liver, kidney, thymus, testis, small intestine and colon.
  • 序列相似性Contains 1 CARD domain.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • 翻译后修饰Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF34 promotes proteasomal degradation and thereby negatively regulates NOD1 for instance in NF-kappa-B activition.
  • 细胞定位Cytoplasm. Cell membrane. Apical cell membrane. Basolateral cell membrane. Detected in the cytoplasm and at the cell membrane. Following bacterial infection, localizes to bacterial entry sites in the cell membrane. Recruited to the basolateral and apical membranes in polarized epithelial cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CARD 4 antibody
    • CARD4 antibody
    • Caspase recruitment domain 4 antibody
    • Caspase recruitment domain containing protein 4 antibody
    • Caspase recruitment domain family member 4 antibody
    • Caspase recruitment domain protein 4 antibody
    • Caspase recruitment domain-containing protein 4 antibody
    • CLR 7.1 antibody
    • CLR7.1 antibody
    • NLR family CARD domain containing 1 antibody
    • NLRC 1 antibody
    • NLRC1 antibody
    • NOD 1 antibody
    • Nod1 antibody
    • NOD1 protein antibody
    • NOD1_HUMAN antibody
    • Nucleotide binding oligomerization domain containing 1 antibody
    • Nucleotide binding oligomerization domain leucine rich repeat and CARD domain containing 1 antibody
    • Nucleotide-binding oligomerization domain-containing protein 1 antibody
    • Protein Nod1 antibody
    see all

Anti-CARD4 antibody 图像

  • All lanes : Anti-CARD4 antibody (ab22143) at 1/2500 dilution

    Lane 1 : CARD4 in recombinant fusion protein containing amino acids 930-949
    Lane 2 : fusion partner without CARD4 amino acids 930-949


    Predicted band size : 106 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 14 kDa,57 kDa. We are unsure as to the identity of these extra bands.

Anti-CARD4 antibody (ab22143)参考文献

This product has been referenced in:
  • Petterson T  et al. Effects of NOD-like receptors in human B lymphocytes and crosstalk between NOD1/NOD2 and Toll-like receptors. J Leukoc Biol 89:177-87 (2011). IHC-P ; Human . Read more (PubMed: 20844241) »
  • Enoksson M  et al. Human cord blood-derived mast cells are activated by the Nod1 agonist M-TriDAP to release pro-inflammatory cytokines and chemokines. J Innate Immun 3:142-9 (2011). Human . Read more (PubMed: 21099203) »

See all 2 Publications for this product

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