概述

  • 产品名称Anti-CARD15抗体[2D9]
    参阅全部 CARD15 一抗
  • 描述
    小鼠单克隆抗体[2D9] to CARD15
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 28-301 (MCS QEAFQAQRSQ LVELLVSGSL EGFESVLDWL LSWEVLSWED YEGFHLLGQP LSHLARRLLD TVWNKGTWAC QKLIAAAQEA QADSQSPKLH GCWDPHSLHP ARDLQSHRPA IVRRLHSHVE NMLDLAWERG FVSQYECDEI RLPIFTPSQR ARRLLDLATV KANGLAAFLL QHVQELPVPL ALPLEAATCK KYMAKLRTTV SAQSRFLSTY DGAETLCLED IYTENVLEVW ADVGMAGPPQ KSPATLGLEE LFSTPGHLND DADTVLVVGE A) of Human CARD15

  • 阳性对照
    • This antibody gave a positive signal when tested against HEK293 overexpressing CARD15 protein.It also gave a positive signal in FFPE human tonsil tissue sections.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号2D9
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab31488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa).
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS).
  • 组织特异性Monocytes-specific.
  • 疾病相关Defects in NOD2 are the cause of Blau syndrome (BS) [MIM:186580]. BS is a rare autosomal dominant disorder characterized by early-onset granulomatous arthritis, uveitis and skin rash.
    Defects in NOD2 are a cause of susceptibility to inflammatory bowel disease type 1 (IBD1) [MIM:266600]. IBD1 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
    Defects in NOD2 are the cause of sarcoidosis early-onset (EOS) [MIM:609464]. EOS is a form of sarcoidosis manifesting in children younger than 4 years of age. Sarcoidosis is an idiopathic, systemic, inflammatory disease characterized by the formation of immune granulomas in involved organs. Granulomas predominantly invade the lungs and the lymphatic system, but also skin, liver, spleen, eyes and other organs may be involved. Early-onset sarcoidosis is quite rare and has a distinct triad of skin, joint and eye disorders, without apparent pulmonary involvement. Compared with an asymptomatic and sometimes naturally disappearing course of the disease in older children, early-onset sarcoidosis is progressive and in many cases causes severe complications, such as blindness, joint destruction and visceral involvement.
  • 序列相似性Contains 2 CARD domains.
    Contains 9 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACUG antibody
    • Arthrocutaneouveal granulomatosis antibody
    • BLAU antibody
    • CARD15 antibody
    • Caspase recruitment domain family, member 15 antibody
    • Caspase recruitment domain protein 15 antibody
    • Caspase recruitment domain-containing protein 15 antibody
    • CD antibody
    • CLR16.3 antibody
    • IBD1 antibody
    • Inflammatory bowel disease protein 1 antibody
    • LRR containing protein antibody
    • NLR family, CARD domain containing 2 antibody
    • NLRC2 antibody
    • NOD like receptor C2 antibody
    • NOD2 antibody
    • NOD2 protein antibody
    • NOD2_HUMAN antibody
    • NOD2B antibody
    • nucleotide binding oligomerization domain 2 antibody
    • Nucleotide binding oligomerization domain containing 2 antibody
    • Nucleotide binding oligomerization domain, leucine rich repeat and CARD domain containing 2 antibody
    • Nucleotide-binding oligomerization domain-containing protein 2 antibody
    • PSORAS1 antibody
    see all

Anti-CARD15 antibody [2D9] 图像

  • Anti-CARD15 antibody [2D9] (ab31488) at 2 µg/ml + HEK 293 overexpressing CARD15 at 20 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 115 kDa
    Observed band size : 115 kDa


    Exposure time : 150 seconds
  • IHC image of CARD15 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31488, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-CARD15 antibody [2D9] (ab31488)参考文献

This product has been referenced in:
  • Zhang YY  et al. Expression and Functional Characterization of NOD2 in Decidual Stromal Cells Isolated during the First Trimester of Pregnancy. PLoS One 9:e99612 (2014). WB, IHC-P ; Human . Read more (PubMed: 24932916) »
  • Magni S  et al. miRNAs Affect the Expression of Innate and Adaptive Immunity Proteins in Celiac Disease. Am J Gastroenterol N/A:N/A (2014). Read more (PubMed: 25070052) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (macrophage)
Specification macrophage
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 22 2014

Application Western blot
Loading amount 1e+006 cells
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (primary human macrophage)
Specification primary human macrophage
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 18 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"