概述

  • 产品名称Anti-CARD11抗体
    参阅全部 CARD11 一抗
  • 描述
    兔多克隆抗体to CARD11
  • 经测试应用适用于: ICC/IF, WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus), Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 275-325 of Human CARD11 (NP_115791.3).

  • 阳性对照
    • Ramos cell lysate and K562 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab91463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 23129749
WB 1/2000 - 1/10000. Predicted molecular weight: 133 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.
  • 组织特异性Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLa S3, Molt-4, A549 and G431 cells.
  • 序列相似性Contains 1 CARD domain.
    Contains 1 guanylate kinase-like domain.
    Contains 1 PDZ (DHR) domain.
  • 翻译后修饰Phosphorylation at Ser-559, Ser-644 and Ser-652 by PRKCBB leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling.
  • 细胞定位Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 0610008L17Rik antibody
    • 2410011D02Rik antibody
    • Bcl10 interacting maguk protein 3 antibody
    • BIMP 3 antibody
    • BIMP3 antibody
    • CAR11_HUMAN antibody
    • CARD 11 antibody
    • CARD containing MAGUK protein 3 antibody
    • Card maguk protein 1 antibody
    • CARD-containing MAGUK protein 1 antibody
    • CARD11 antibody
    • CARD11 protein antibody
    • Carma 1 antibody
    • CARMA1 antibody
    • Caspase recruitment domain containing protein 11 antibody
    • Caspase recruitment domain family member 11 antibody
    • Caspase recruitment domain-containing protein 11 antibody
    • MGC133069 antibody
    see all

Anti-CARD11 antibody 图像

  • All lanes : Anti-CARD11 antibody (ab91463) at 0.1 µg/ml

    Lane 1 : Ramos cell lysate at 50 µg
    Lane 2 : Ramos cell lysate at 15 µg
    Lane 3 : Ramos cell lysate at 5 µg
    Lane 4 : K562 cell lysate at 50 µg


    Predicted band size : 133 kDa
  • Sample: whole cell lysate from Ramos cells (1 mg for IP, 20% of IP loaded) .
    Antibody: ab91463 at 1 µg/ml for WB and at 10 µg/mg lysate for IP.
    Detection: chemiluminescence with exposure times of 10 seconds.

Anti-CARD11 antibody (ab91463)参考文献

This product has been referenced in:
  • Snow AL  et al. Congenital B cell lymphocytosis explained by novel germline CARD11 mutations. J Exp Med 209:2247-61 (2012). ICC/IF ; Human . Read more (PubMed: 23129749) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab91463.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"