概述

  • 产品名称
  • 描述
    山羊多克隆抗体to CARD11
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    QRKTIWVDEDQL

    with a Cysteine residue linker, corresponding to C terminal amino acids 1143-1154 of Human CARD11.

  • 阳性对照
    • Human spleen tissue; Jurkat cell lysate. The pattern of staining with this antibody is similar to that observed with ab189167 and ab113409 in that the reagent stains macrophages, mast cells, subsets of lymphocytes and plasma cells in various organs across the body. It also stains adrenal cortex, heart, kidney, small intestine epithelium, skeletal muscle, and pancreatic exocrine epithelium and Kupffer cells in the liver

性能

应用

Our Abpromise guarantee covers the use of ab115471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 133 kDa.
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.
  • 组织特异性
    Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLa S3, Molt-4, A549 and G431 cells.
  • 序列相似性
    Contains 1 CARD domain.
    Contains 1 guanylate kinase-like domain.
    Contains 1 PDZ (DHR) domain.
  • 翻译后修饰
    Phosphorylation at Ser-559, Ser-644 and Ser-652 by PRKCBB leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling.
  • 细胞定位
    Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 0610008L17Rik antibody
    • 2410011D02Rik antibody
    • Bcl10 interacting maguk protein 3 antibody
    • BIMP 3 antibody
    • BIMP3 antibody
    • CAR11_HUMAN antibody
    • CARD 11 antibody
    • CARD containing MAGUK protein 3 antibody
    • Card maguk protein 1 antibody
    • CARD-containing MAGUK protein 1 antibody
    • CARD11 antibody
    • CARD11 protein antibody
    • Carma 1 antibody
    • CARMA1 antibody
    • Caspase recruitment domain containing protein 11 antibody
    • Caspase recruitment domain family member 11 antibody
    • Caspase recruitment domain-containing protein 11 antibody
    • MGC133069 antibody
    see all

图片

  • Anti-CARD11 antibody (ab115471) at 1 µg/ml + Jurkat cell lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 133 kDa
  • ab115471 at 2ug/ml staining CARD11 in Formalin-fixed, Paraffin-embedded Human spleen tissue by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

文献

ab115471 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab115471.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册