Anti-Carcino Embryonic Antigen CEA抗体[NCRC16 (AKA 161)] (ab22705)

概述

  • 产品名称
    Anti-Carcino Embryonic Antigen CEA抗体[NCRC16 (AKA 161)]
    参阅全部 Carcino Embryonic Antigen CEA 一抗
  • 描述
    小鼠单克隆抗体[NCRC16 (AKA 161)] to Carcino Embryonic Antigen CEA
  • 特异性
    Cross reacts with a similar epitope on NCA.
  • 经测试应用
    适用于: ICC/IF, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Colorectal cancer cell lines (Human).

  • 阳性对照
    • LS174 T cells, colon carcinoma IHC-P: FFPE human colon tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab22705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.
  • 组织特异性
    Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
  • 序列相似性
    Belongs to the immunoglobulin superfamily. CEA family.
    Contains 7 Ig-like (immunoglobulin-like) domains.
  • 翻译后修饰
    Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Carcinoembryonic antigen antibody
    • Carcinoembryonic antigen-related cell adhesion molecule 5 antibody
    • CD66e antibody
    • CEA antibody
    • Ceacam5 antibody
    • CEAM5_HUMAN antibody
    • DKFZp781M2392 antibody
    • Meconium antigen 100 antibody
    • OTTHUMP00000199032 antibody
    • OTTHUMP00000199033 antibody
    • OTTHUMP00000199034 antibody
    see all

图片

  • IHC image of Carcino Embryonic Antigen CEA staining in human colon formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22705, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab22705 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22705, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Durrant LG  et al. Enhanced recognition of human colorectal tumour cells using combinations of monoclonal antibodies. Br J Cancer 60:855-60 (1989). Read more (PubMed: 2481485) »

See 1 Publication for this product

客户评价及客户问答

Thank you for submitting an Abreview of ab22705. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody for trying it in WB, we would like to follow up on thisto seeif w...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Recombinant protein (recombinant protein)
Loading amount
0.02 µg
Specification
recombinant protein
Gel Running Conditions
Reduced Denaturing (gel 10%)
Blocking step
Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Mar 13 2012

Application
ELISA
Sample
Human Purified protein (not sure)
Specification
not sure
Type
Sandwich (Capture)
Username

Abcam user community

Verified customer

提交于 Jan 07 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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