Anti-Carcino Embryonic Antigen CEA抗体[C6G9] (ab11330)

概述

  • 产品名称Anti-Carcino Embryonic Antigen CEA抗体[C6G9]
    参阅全部 Carcino Embryonic Antigen CEA 一抗
  • 描述
    小鼠单克隆抗体[C6G9] to Carcino Embryonic Antigen CEA
  • 特异性Monoclonal Anti-Human Carcinoembryonic Antigen (CEA) reacts specifically with human CEA (CD66e, 180 kDa) from several types of malignant tissues including colorectal, lung and breast tumors. It also stains medullary carcinomas of the thyroid. The product reacts strongly with the cell surface and cytoplasm of malignant glands in colorectal adenocarcinomas. A weak reactivity is seen with normal colon mucosa and occasionally with bile canalicular and pancreatic acinar cells, but not with other tested normal tissues including term placenta.
  • 经测试应用适用于: Dot blot, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    CEA isolated from a human colon adenocarcinoma cell line.

  • 常规说明Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

性能

应用

Our Abpromise guarantee covers the use of ab11330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot
WB
IHC-P
  • 应用说明Dot: 1/8000.
    IHC-P: 1/8000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
    WB: 1/8000. Predicted molecular weight: 180 kDa.

    There is no cross reactivity with nonspecific cross-reacting antigen (NCA) present in granulocytes by immunoblotting, flow cytometry or immunohistochemical techniques.

    The antibody recognizes an epitope that is resistant to 30 minute oxidation by 1% sodium periodate solution in routine formalin-fixed, paraffin-embedded tissue sections. Enzymatic predigestion with proteolytic enzymes enhances immunohistochemical staining of formalinfixed, paraffin-embedded tissue sections with the antibody.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.
    • 组织特异性Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
    • 序列相似性Belongs to the immunoglobulin superfamily. CEA family.
      Contains 7 Ig-like (immunoglobulin-like) domains.
    • 翻译后修饰Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.
    • 细胞定位Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Carcinoembryonic antigen antibody
      • Carcinoembryonic antigen-related cell adhesion molecule 5 antibody
      • CD66e antibody
      • CEA antibody
      • Ceacam5 antibody
      • CEAM5_HUMAN antibody
      • DKFZp781M2392 antibody
      • Meconium antigen 100 antibody
      • OTTHUMP00000199032 antibody
      • OTTHUMP00000199033 antibody
      • OTTHUMP00000199034 antibody
      see all

    Anti-Carcino Embryonic Antigen CEA antibody [C6G9] (ab11330)参考文献

    This product has been referenced in:
    • Guo X  et al. In vitro evaluation of cancer-specific NF-kappaB-CEA enhancer-promoter system for 5-fluorouracil prodrug gene therapy in colon cancer cell lines. Br J Cancer 97:745-54 (2007). WB ; Human . Read more (PubMed: 17687334) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry. For the following antibodies, epitope specificity is given per ISOBM classification and we have only following information: Ab10038 - Epitope specificity group IV, cross-reactive with NCA2. Ab10039 - Epitope specificity ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"