Anti-Carcino Embryonic Antigen CEA抗体[26/3/13] (ab4451)

概述

  • 产品名称Anti-Carcino Embryonic Antigen CEA抗体[26/3/13]
    参阅全部 Carcino Embryonic Antigen CEA 一抗
  • 描述
    小鼠单克隆抗体[26/3/13] to Carcino Embryonic Antigen CEA
  • 特异性This antibody recognises exclusively CEACAM 5 transiently expressed on the cell surface of transfected BOSC cells. It can be used to distinguish CEACAM 5 from all other CEACAM and probably all pregnancy-specific glycoproteins molecules, namely CEACAM 1 (BGP/CD66a), CEACAM 3 (CGM1/CD66d), CEACAM 4 (CGM7), CEACAM 6 (NCA/CD66c), CEACAM 7 (CGM2), CEACAM 8 (CGM6/CD66b)and PSG1 (CD66f) based on its reactivity pattern with stable HeLa transfectants expressing individual CEA family members. This antibody was included and characterized in the studies from the VIth Leucocyte Typing Workshop (Grunert F et al. 1994)
  • 经测试应用适用于: IHC-P, Sandwich ELISA, ELISA, Flow Cyt, WB, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native protein (partially purified) (Human) from a perchloric acid extract from liver metastases of colonic tumors (Grunert F, et al. 1985)

  • 阳性对照
    • IHC-P: human normal colon tissue sections
  • 常规说明Antibodies produced from cDNA: Conventional technologies usually either generate antibodies against purified proteins, or against synthetic peptides based on amino acid sequences derived from DNA sequence data. Genetic immunization involves introducing the gene in the form of a cDNA directly into an animal which translates this cDNA into protein thus stimulating an immune response against the foreign protein. Although the synthetic peptide approach is comparable in speed, the quality of antibodies generated by genetic immunization is far superior. This is because the protein is made by the immunized animal, utilzing complex cellular mechanisms that allow it to gain a native conformation. Antibodies are then generated against a native protein, such as is found in the blood or tissues of its host species. Membrane-bound or secreted proteins often create problems for conventional antibody technology because in their native form, they are often modified by glycosylation, or in some cases exist as multiple membrane-spanning proteins that are not soluble following isolation or synthesis in recombinant systems. All of these problems are avoided if the immunized animal makes the protein itself. Antibodies generated by genetic immunization have been shown to have binding affinities to the protein in the sub-nanomolar range, which are approximately 100x higher than conventionally developed antibodies and much higher than single chain antibodies. Results confirm published data for much higher avidity of sera generated by genetic immunization as compared with that gained by immunization with a corresponding recombinant protein.

性能

应用

Our Abpromise guarantee covers the use of ab4451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA Use a concentration of 0.2 µg/ml.

For sandwich ELISA, use this antibody as Capture at 0.2µg/ml

ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 84 kDa.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.
  • 组织特异性Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
  • 序列相似性Belongs to the immunoglobulin superfamily. CEA family.
    Contains 7 Ig-like (immunoglobulin-like) domains.
  • 翻译后修饰Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Carcinoembryonic antigen antibody
    • Carcinoembryonic antigen-related cell adhesion molecule 5 antibody
    • CD66e antibody
    • CEA antibody
    • Ceacam5 antibody
    • CEAM5_HUMAN antibody
    • DKFZp781M2392 antibody
    • Meconium antigen 100 antibody
    • OTTHUMP00000199032 antibody
    • OTTHUMP00000199033 antibody
    • OTTHUMP00000199034 antibody
    see all

Anti-Carcino Embryonic Antigen CEA antibody [26/3/13] 图像

  • Standard Curve for Carcino Embryonic Antigen CEA dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [26/3/13] to Carcino Embryonic Antigen CEA (ab4451) at 0.2ug/ml and Detector Antibody Rabbit polyclonal to Carcino Embryonic Antigen CEA (ab15987) at 0.5ug/ml
  • IHC image of Carcino Embryonic Antigen CEA staining in human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4451, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

实验方案

Anti-Carcino Embryonic Antigen CEA antibody [26/3/13] (ab4451)参考文献

This product has been referenced in:
  • Stern LA  et al. Geometry and expression enhance enrichment of functional yeast-displayed ligands via cell panning. Biotechnol Bioeng 113:2328-41 (2016). Read more (PubMed: 27144954) »
  • Ostendorff HP  et al. Multiplexed VeraCode bead-based serological immunoassay for colorectal cancer. J Immunol Methods 400-401:58-69 (2013). Human . Read more (PubMed: 24161315) »

See all 4 Publications for this product

Product Wall

Thank you for your email.
Yes, that is correct. Alternatively, you can just use a secondary antibody that has already been conjugated with HRP. We carry many in our catalog. Please let me know if you need help selecting one.
I hope this infor...

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The conjugation kit ab102891 is compatible with antibody formulations that contain up to 1% BSA, even though the BSA is in 10-fold excess to the antibody (10mg/ml BSA compared to 1 mg/ml IgG for ab71720). Some of the BSA will itself will be conjugated,...

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I think the CEA antibody formulation will be compatible with the other company's labeling kit but you should contact them and send them the buffer components. Here are the components of the ab15987 buffer:

Preservative: 15mM Sodium Azide Con...

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Thank you for your resposne.

As my colleauge has explained in the previous e-mail, our antibodies and proteins will regrettably not have been tested and guaranteed in the application you are using (i.e. electrical biosensors).

As EL...

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Thank you for your enquiry.

I can confirm that ab4451 and ab15987 have been tested and guarantee for use in sELISA together according to the datasheets. Theab742 Carcino Embryonic Antigen CEA protein is full length native protein so should b...

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For the CEA assay, I recommend the antibodies I mentioned in my previous e-mail, ab4451 for capture and ab15987 for detection, followed by the secondary, anti-rabbit IgG conjugated to HRP, ab97080, and appropriate substrate, for instance, TMB.
For...

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Thank you for contacting us. I will be happy to make some suggestions. Can you please confirm that your samples are human-derived, and that you are interested in a sandwich ELISA, rather than direct or indirect ELISA?

For an example of a san...

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Thank you for your inquiry.


You are inquiring about the sELISA pair, ab4451 for capture and ab15987 for detection, correct?


Currently we do not offer a biotinylated form of ab15987. If you are looking to order this antib...

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Application ELISA
Sample Human Purified protein (not sure)
Specification not sure
Type Sandwich (Capture)
Username

Abcam user community

Verified customer

提交于 Jan 11 2011

Thank you for your enquiry. CEACAM5 [26/3/13]ab4451 belongs to the GOLD1 inhibition group. However, it does not inhibit all other GOLD1 members, and it does not bind to the isolated N-terminal domain of CEACAM5 if expressed alone. Unfortunately, this a...

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