Anti-Carboxypeptidase Y抗体[10A5B5] (ab113685)
Key features and details
- Mouse monoclonal [10A5B5] to Carboxypeptidase Y
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG1
概述
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产品名称
Anti-Carboxypeptidase Y抗体[10A5B5]
参阅全部 Carboxypeptidase Y 一抗 -
描述
小鼠单克隆抗体[10A5B5] to Carboxypeptidase Y -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Saccharomyces cerevisiae -
免疫原
Full length native protein (purified). This information is considered to be commercially sensitive.
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阳性对照
- Yeast membranes
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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纯度
Proprietary Purification -
纯化说明
Near homogeneity (Purity >95% by SDS-PAGE). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation. -
克隆
单克隆 -
克隆编号
10A5B5 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab113685于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.5 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
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说明 |
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WB
Use a concentration of 0.5 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa). |
靶标
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功能
Involved in degradation of small peptides. Digests preferentially peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in P1 position of ester substrate. -
序列相似性
Belongs to the peptidase S10 family. -
翻译后修饰
Enters the endoplasmic reticulum as an inactive zymogen and is modified by four N-linked core oligosaccharides, giving rise to a precursor known as P1 (67 kDa). As P1 transits through the Golgi, extension of its core oligosaccharides leads to the Golgi-modified P2 precursor (69 kDa). P2 is sorted away from secretory proteins at or beyond a late Golgi compartment and is subsequently delivered to the vacuole via a prevacuolar endosome-like compartment. Upon arrival in the vacuole, the N-terminal prosegment of P2 is cleaved to yield the enzymatically active mature vacuolar form of CPY (61 kDa). -
细胞定位
Vacuole. Lysosome-like vacuoles. - Information by UniProt
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数据库链接
- Entrez Gene: 855343 Saccharomyces cerevisiae
- SwissProt: P00729 Saccharomyces cerevisiae
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别名
- carboxypeptidase C PRC1 antibody
- Carboxypeptidase Y antibody
- Carboxypeptidase YSCY antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab113685 被引用在 7 文献中.
- Sun D et al. The cryo-EM structure of the SNX-BAR Mvp1 tetramer. Nat Commun 11:1506 (2020). PubMed: 32198400
- Yanguas F et al. Ent3 and GGA adaptors facilitate diverse anterograde and retrograde trafficking events to and from the prevacuolar endosome. Sci Rep 9:10747 (2019). PubMed: 31341193
- Aviram N et al. The SND proteins constitute an alternative targeting route to the endoplasmic reticulum. Nature 540:134-138 (2016). PubMed: 27905431
- Ast T et al. A cytosolic degradation pathway, prERAD, monitors pre-inserted secretory pathway proteins. J Cell Sci 127:3017-23 (2014). WB ; Saccharomyces cerevisiae . PubMed: 24849653
- Manandhar SP et al. Saccharomyces cerevisiae Env7 is a novel serine/threonine kinase 16-related protein kinase and negatively regulates organelle fusion at the lysosomal vacuole. Mol Cell Biol 33:526-42 (2013). PubMed: 23166297
- Chen J et al. Accumulation of an antidepressant in vesiculogenic membranes of yeast cells triggers autophagy. PLoS One 7:e34024 (2012). PubMed: 22529904
- Li L et al. A Role for Iron-Sulfur Clusters in the Regulation of Transcription Factor Yap5-dependent High Iron Transcriptional Responses in Yeast. J Biol Chem 287:35709-21 (2012). WB ; Saccharomyces cerevisiae . PubMed: 22915593