Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Concentration information loading...
Ammonium Sulphate Precipitation
ab85251 prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/50 - 1/100. Predicted molecular weight: 61 kDa.
The 83 kDa subunit binds and stabilizes the catalytic subunit at 37 degrees Celsius and keeps it in circulation. Under some circumstances it may be an allosteric modifier of the catalytic subunit.
Contains 12 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Whether or not any Cys residues participate in intrachain bonds is unknown, but they do not form interchain disulfide bonds with the 50 kDa catalytic subunit.
Information by UniProt
- ACBP antibody
- Carboxypeptidase N 83 kDa chain antibody
- Carboxypeptidase N large subunit antibody
Anti-Carboxypeptidase N subunit 2 antibody - N-terminal 图像
Western blot - Carboxypeptidase N subunit 2 antibody - N-terminal (ab85251)
Anti-Carboxypeptidase N subunit 2 antibody - N-terminal (ab85251) at 1/50 dilution + Jurkat cell line lysate at 35 µgPredicted band size :
61 kDaObserved band size :
57 kDa (why is the actual band size different from the predicted?
)Additional bands at :
32 kDa,38 kDa,50 kDa. We are unsure as to the identity of these extra bands.
Anti-Carboxypeptidase N subunit 2 antibody - N-terminal (ab85251)参考文献
has not yet been referenced specifically in any publications.
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