Anti-Carboxypeptidase H抗体(ab84631)

概述

  • 产品名称
    Anti-Carboxypeptidase H抗体
    参阅全部 Carboxypeptidase H 一抗
  • 描述
    兔多克隆抗体to Carboxypeptidase H
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Cat, Pig, Chimpanzee, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 36-85 (GMRRRRRLQQ EDGISFEYHR YPELREALVS VWLQCTAISR IYTVGRSFEG) of Human Carboxypeptidase H (NP_001864).

  • 阳性对照
    • MCF7 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab84631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin.
  • 组织特异性
    Isoform 2, but not isoform 1, is overexpressed in hepatocellular carcinoma (at protein level), as well as in other tumors, including pheochromocytomas and paragangliomas.
  • 序列相似性
    Belongs to the peptidase M14 family.
  • 细胞定位
    Nucleus and Secreted. Secretory granules of pancreatic islets, adrenal gland, pituitary and brain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Carboxypeptidase E antibody
    • Carboxypeptidase H antibody
    • Carboxypeptidase H precursor antibody
    • CarboxypeptidaseE antibody
    • CarboxypeptidaseH antibody
    • CBPE_HUMAN antibody
    • Cobalt stimulated chromaffin granule carboxypeptidase antibody
    • CPE antibody
    • Cph 1 antibody
    • CPH antibody
    • Enkephalin convertase antibody
    • Entephalin convertase antibody
    • Insulin granule associated carboxypeptidase antibody
    • Prohormone processing carboxypeptidase antibody
    • Prohormone-processing carboxypeptidase antibody
    see all

Anti-Carboxypeptidase H antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling Carboxypeptidase H with ab84631 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of cell bodies and processes of pinealocytes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - Carboxypeptidase H. Right - Merge.
  • Anti-Carboxypeptidase H antibody (ab84631) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    12% gel
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain, cerebellum tissue labelling Carboxypeptidase H with ab84631 at 5µg/ml.

Anti-Carboxypeptidase H antibody (ab84631)参考文献

ab84631 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab84631.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册