Anti-Carboxypeptidase H抗体(ab84631)

概述

  • 产品名称Anti-Carboxypeptidase H抗体
    参阅全部 Carboxypeptidase H 一抗
  • 描述
    兔多克隆抗体to Carboxypeptidase H
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Cat, Pig, Chimpanzee, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 36-85 (GMRRRRRLQQ EDGISFEYHR YPELREALVS VWLQCTAISR IYTVGRSFEG) of Human Carboxypeptidase H (NP_001864).

  • 阳性对照
    • MCF7 cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab84631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-Carboxypeptidase H antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling Carboxypeptidase H with ab84631 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of cell bodies and processes of pinealocytes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - Carboxypeptidase H. Right - Merge.
  • Anti-Carboxypeptidase H antibody (ab84631) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    12% gel
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain, cerebellum tissue labelling Carboxypeptidase H with ab84631 at 5µg/ml.

Anti-Carboxypeptidase H antibody (ab84631)参考文献

ab84631 has not yet been referenced specifically in any publications.

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