概述

  • 产品名称Anti-Carboxypeptidase H抗体
    参阅全部 Carboxypeptidase H 一抗
  • 描述
    兔多克隆抗体to Carboxypeptidase H
  • 经测试应用适用于: ICC, WB, IP, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human, Monkey
  • 免疫原

    Synthetic peptide (Human) conjugated to KLH (C terminal).

  • 阳性对照
    • Adrenal gland.
  • 常规说明This product should be stored undiluted. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch.

性能

应用

Our Abpromise guarantee covers the use of ab11044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC 1/100 - 1/400.
WB 1/500 - 1/2000. Predicted molecular weight: 53 kDa.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin.
  • 组织特异性Isoform 2, but not isoform 1, is overexpressed in hepatocellular carcinoma (at protein level), as well as in other tumors, including pheochromocytomas and paragangliomas.
  • 序列相似性Belongs to the peptidase M14 family.
  • 细胞定位Nucleus and Secreted. Secretory granules of pancreatic islets, adrenal gland, pituitary and brain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Carboxypeptidase E antibody
    • Carboxypeptidase H antibody
    • Carboxypeptidase H precursor antibody
    • CarboxypeptidaseE antibody
    • CarboxypeptidaseH antibody
    • CBPE_HUMAN antibody
    • Cobalt stimulated chromaffin granule carboxypeptidase antibody
    • CPE antibody
    • Cph 1 antibody
    • CPH antibody
    • Enkephalin convertase antibody
    • Entephalin convertase antibody
    • Insulin granule associated carboxypeptidase antibody
    • Prohormone processing carboxypeptidase antibody
    • Prohormone-processing carboxypeptidase antibody
    see all

Anti-Carboxypeptidase H antibody 图像

  • ab11044 staining Carboxypeptidase H in murine pancreatic tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using 10mM citrate buffer pH 6.0. Samples were then blocked with 5% serum for 1 hour at 20°C followed by incubation with the primary antibody at a 1/800 dilution for 16 hours at 4°C. A Cy2®-conjugated donkey anti-rabbit polyclonal was used as secondary antibody at a 1/200 dilution.

    See Abreview

Anti-Carboxypeptidase H antibody (ab11044)参考文献

This product has been referenced in:
  • Meliopoulos VA  et al. MicroRNA regulation of human protease genes essential for influenza virus replication. PLoS One 7:e37169 (2012). WB ; Human . Read more (PubMed: 22606348) »
  • Parkinson D Carboxypeptidase H in bovine pituitary gland: soluble forms are not processed at the C-terminus. Mol Cell Endocrinol 86:221-33 (1992). Read more (PubMed: 1511790) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (pancreas)
Specification pancreas
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mm citrate buffer PH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Nov 12 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"