Anti-Carboxymethyl Lysine抗体[NF-1G] (ab30917)

概述

  • 产品名称
    Anti-Carboxymethyl Lysine抗体[NF-1G]
    参阅全部 Carboxymethyl Lysine 一抗
  • 描述
    小鼠单克隆抗体[NF-1G] to Carboxymethyl Lysine
  • 特异性
    This antibody is suitable for the detection of N Epsilon (Carboxymethyl) Lysine (CML) in tissues and tissue extracts.
  • 经测试应用
    适用于: ELISA, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Species independent
  • 免疫原

    N Epsilon (Carboxymethyl) Lysine (CML)-HSA.

性能

应用

Our Abpromise guarantee covers the use of ab30917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use a concentration of 0.1 - 0.5 µg/ml.
IHC-Fr Use a concentration of 2 µg/ml.
IHC-P Use at an assay dependent concentration.

靶标

  • 相关性
    N epsilon carboxymethyl lysine (CML or Carboxymethyl Lysine) is formed by the non enzymatic Schiff base reaction of glucose with proteins, followed by an Amadori rearrangement and oxidation that leaves only a carboxymethyl group attached to the lysine. The levels of CML adducts accumulate over time and have been used as an indicator of both serum glucose levels and oxidative protein damage. Elevated serum CML modified proteins have been associated with diabetes and may contribute to diabetic retinopathy, nephropathy and angiopathy.
  • 别名
    • CML antibody
    • N Epsilon (Carboxymethyl) Lysine antibody

图片

  • ab30917 staining Carboxymethyl Lysine in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formalin and embedded in paraffin. Samples were incubated with primary antibody (1/125 in PBS + 1% BSA) for overnight at 4°C. A Biotin-conjugated secondary was used with DAB visualisation the samples were then counterstained with haematoxylin.

文献

This product has been referenced in:
  • Crisan M  et al. Expression of Advanced Glycation End-Products on Sun-Exposed and Non-Exposed Cutaneous Sites during the Ageing Process in Humans. PLoS One 8:e75003 (2013). IHC-P ; Human . Read more (PubMed: 24116020) »

See 1 Publication for this product

客户评价及客户问答

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Mouse Tissue lysate - whole (cortex and hippocampus)
Specification
cortex and hippocampus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RM°C
Username

Abcam user community

Verified customer

提交于 Mar 28 2014

I'm sorry I was not clearer in my explanation. Both ab30917 and ab30922 should be suitable for you experiment. The reason why we sometimes have what seems to be duplicates within our catalogue is that in order to make sure we have a continuous supply o...

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Thank you for contacting us. The two antibodies (ab30917 and ab30922) are both mouse monoclonal antibodies directed against N Epsilon (Carboxymethyl) Lysine (CML). They have both been tested with ELISA, IHP (frozen sections and paraffin). They ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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