Anti-Carboxymethyl Lysine抗体[CML26] (ab125145)


  • 产品名称Anti-Carboxymethyl Lysine抗体[CML26]
    参阅全部 Carboxymethyl Lysine 一抗
  • 描述
    小鼠单克隆抗体[CML26] to Carboxymethyl Lysine
  • 经测试应用适用于: WB, IHC-P, IHC-Fr, ICC/IF, ELISA, Flow Cytmore details
  • 种属反应性
    与反应: Species independent
  • 免疫原

    Carboxymethyl Lysine conjugated to KLH.

  • 阳性对照
    • Human cardiac tissue (intramyocardial arteries).
  • 常规说明Other isotypes maybe present.



Our Abpromise guarantee covers the use of ab125145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.
IHC-P 1/50. Fix with 4% formalin.
IHC-Fr 1/50.
ICC/IF Use at an assay dependent concentration. Fix with 2% phosphate-buffered glutaraldehyde solution.
ELISA Use at an assay dependent concentration.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • 相关性N epsilon carboxymethyl lysine (CML or Carboxymethyl Lysine) is formed by the non enzymatic Schiff base reaction of glucose with proteins, followed by an Amadori rearrangement and oxidation that leaves only a carboxymethyl group attached to the lysine. The levels of CML adducts accumulate over time and have been used as an indicator of both serum glucose levels and oxidative protein damage. Elevated serum CML modified proteins have been associated with diabetes and may contribute to diabetic retinopathy, nephropathy and angiopathy.
  • 别名
    • CML antibody
    • N Epsilon (Carboxymethyl) Lysine antibody

Anti-Carboxymethyl Lysine antibody [CML26] 图像

  • Overlay histogram showing HepG2 cells stained with ab125145 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab125145, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Carboxymethyl Lysine antibody [CML26] (ab125145)参考文献

This product has been referenced in:
  • Lo MC  et al. Ne-(carboxymethyl) lysine-induced mitochondrial fission and mitophagy cause decreased insulin secretion from ß-cells. Am J Physiol Endocrinol Metab 309:E829-39 (2015). Read more (PubMed: 26394662) »

See 1 Publication for this product

Product Wall

The protein band size of western blot depends on which protein has adducted by CML.
Carboxymethyl Lysine (CML) is an advanced glycation end product found on proteins and lipids as a result of oxidative stress and chemical glycation. CML adducts acc...

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