Anti-Carboxymethyl Lysine抗体[CML26] (ab125145)
Key features and details
- Mouse monoclonal [CML26] to Carboxymethyl Lysine
- Suitable for: WB, IHC-P, IHC-Fr, ICC/IF, ELISA, Flow Cyt
- Reacts with: Species independent
- Isotype: IgG1
概述
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产品名称
Anti-Carboxymethyl Lysine抗体[CML26]
参阅全部 Carboxymethyl Lysine 一抗 -
描述
小鼠单克隆抗体[CML26] to Carboxymethyl Lysine -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, IHC-Fr, ICC/IF, ELISA, Flow Cytmore details -
种属反应性
与反应: Species independent -
免疫原
Carboxymethyl Lysine conjugated to KLH.
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阳性对照
- Human cardiac tissue (intramyocardial arteries).
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常规说明
Other isotypes maybe present.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
CML26 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125145于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration.
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IHC-P |
1/50.
Fix with 4% formalin. |
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IHC-Fr |
1/50.
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ICC/IF |
Use at an assay dependent concentration.
Fix with 2% phosphate-buffered glutaraldehyde solution. |
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ELISA |
Use at an assay dependent concentration.
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Flow Cyt |
Use 0.1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
Use at an assay dependent concentration. |
IHC-P
1/50. Fix with 4% formalin. |
IHC-Fr
1/50. |
ICC/IF
Use at an assay dependent concentration. Fix with 2% phosphate-buffered glutaraldehyde solution. |
ELISA
Use at an assay dependent concentration. |
Flow Cyt
Use 0.1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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相关性
N epsilon carboxymethyl lysine (CML or Carboxymethyl Lysine) is formed by the non enzymatic Schiff base reaction of glucose with proteins, followed by an Amadori rearrangement and oxidation that leaves only a carboxymethyl group attached to the lysine. The levels of CML adducts accumulate over time and have been used as an indicator of both serum glucose levels and oxidative protein damage. Elevated serum CML modified proteins have been associated with diabetes and may contribute to diabetic retinopathy, nephropathy and angiopathy. -
别名
- CML antibody
- N Epsilon (Carboxymethyl) Lysine antibody
图片
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Overlay histogram showing HepG2 cells stained with ab125145 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab125145, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab125145 被引用在 10 文献中.
- Castro B et al. SCA® Slows the Decline of Functional Parameters Associated with Senescence in Skin Cells. Int J Mol Sci 23:N/A (2022). PubMed: 35742982
- Wang ZQ & Sun Z Dietary Nε-(carboxymethyl) lysine affects cardiac glucose metabolism and myocardial remodeling in mice. World J Diabetes 13:972-985 (2022). PubMed: 36437860
- Altomare A et al. In-Depth AGE and ALE Profiling of Human Albumin in Heart Failure: Ex Vivo Studies. Antioxidants (Basel) 10:N/A (2021). PubMed: 33673523
- Jing L et al. Role of Sortilin and Matrix Vesicles in Ne-Carboxymethyl-Lysine-Induced Diabetic Atherosclerotic Calcification. Diabetes Metab Syndr Obes 13:4141-4151 (2020). PubMed: 33177854
- Rosenstock P et al. Glycation interferes with natural killer cell function. Mech Ageing Dev 178:64-71 (2019). PubMed: 30659859
- Dubey NK et al. Adipose-derived Stem Cells Attenuates Diabetic Osteoarthritis via Inhibition of Glycation-mediated Inflammatory Cascade. Aging Dis 10:483-496 (2019). PubMed: 31164994
- Nevin C et al. Investigating the Glycating Effects of Glucose, Glyoxal and Methylglyoxal on Human Sperm. Sci Rep 8:9002 (2018). PubMed: 29899461
- Frescas D et al. Senescent cells expose and secrete an oxidized form of membrane-bound vimentin as revealed by a natural polyreactive antibody. Proc Natl Acad Sci U S A 114:E1668-E1677 (2017). WB ; Mouse . PubMed: 28193858
- Aitken RJ et al. Involvement of homocysteine, homocysteine thiolactone, and paraoxonase type 1 (PON-1) in the etiology of defective human sperm function. Andrology 4:345-60 (2016). PubMed: 26825875
- Lo MC et al. Ne-(carboxymethyl) lysine-induced mitochondrial fission and mitophagy cause decreased insulin secretion from ß-cells. Am J Physiol Endocrinol Metab 309:E829-39 (2015). PubMed: 26394662