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Our Abpromise guarantee covers the use of ab27684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/2500 - 1/20000.|
|WB||1/2500 - 1/20000.|
|Conjugation||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
ab27684 staining Carboxymethyl Lysine in Mouse lung tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/1000) for 12 hours at 4°C. A biotin-conjugated anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Chris MattisonBlocking Step: 2% Milk for 30 minutes at 25°C
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