Anti-Carboxymethyl Lysine抗体(ab27684)

概述

  • 产品名称
    Anti-Carboxymethyl Lysine抗体
    参阅全部 Carboxymethyl Lysine 一抗
  • 描述
    兔多克隆抗体to Carboxymethyl Lysine
  • 特异性
    This antibody specifically binds to Carboxy methyl Lysine modified proteins.
  • 经测试应用
    适用于: ELISA, WB, Conjugation, IHC-Frmore details
  • 种属反应性
    与反应: Species independent
  • 免疫原

    Carboxy methyl Lysine conjugated to KLH.

性能

应用

Our Abpromise guarantee covers the use of ab27684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/2500 - 1/20000.
WB 1/2500 - 1/20000.
Conjugation Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 相关性
    N epsilon carboxymethyl lysine (CML or Carboxymethyl Lysine) is formed by the non enzymatic Schiff base reaction of glucose with proteins, followed by an Amadori rearrangement and oxidation that leaves only a carboxymethyl group attached to the lysine. The levels of CML adducts accumulate over time and have been used as an indicator of both serum glucose levels and oxidative protein damage. Elevated serum CML modified proteins have been associated with diabetes and may contribute to diabetic retinopathy, nephropathy and angiopathy.
  • 别名
    • CML antibody
    • N Epsilon (Carboxymethyl) Lysine antibody

Anti-Carboxymethyl Lysine antibody 图像

  • All lanes : Anti-Carboxymethyl Lysine antibody (ab27684) at 1/1000 dilution (in PBST for 18 hours at 4°C)

    Lane 1 : Whole tissue lysate of raw cashew nuts
    Lane 2 : Whole tissue lysate of roasted cashew nuts

    Lysates/proteins at 50 µg per lane.

    Secondary
    An HRP-conjugated Goat anti-rabbit polyclonal
    Developed using the ECL technique

    Observed band size : 10-12 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Chris Mattison

    Blocking Step: 2% Milk for 30 minutes at 25°C
  • ab27684 staining Carboxymethyl Lysine in Mouse lung tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/1000) for 12 hours at 4°C. A biotin-conjugated anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

Anti-Carboxymethyl Lysine antibody (ab27684)参考文献

This product has been referenced in:
  • Ying G  et al. Effect of a local, one time, low-dose injection of zoledronic acid on titanium implant osseointegration in ovariectomized rats. Arch Med Sci 12:941-949 (2016). IHC-P . Read more (PubMed: 27695483) »
  • Machahua C  et al. Increased AGE-RAGE ratio in idiopathic pulmonary fibrosis. Respir Res 17:144 (2016). WB ; Human . Read more (PubMed: 27816054) »

See all 9 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Mouse Tissue sections (Lung)
Specification
Lung
Permeabilization
Yes - Triton X100 0.5%
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 13 2013

Thanks for your call earlier this week and for your patience. I have looked through some literature, and I think the best positive control for these markers that we will have are the human squamous cell carcinoma slides ab5107- http://www.abcam.com/Ski...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Cashew nuts (Anacardiaceae) Tissue lysate - whole (whole nut)
Loading amount
50 µg
Specification
whole nut
Treatment
roasting
Gel Running Conditions
Reduced Denaturing (4-20)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Mr. Chris Mattison

Verified customer

提交于 Oct 18 2011

Thank you for your call yesterday and for our conversation about positive control to use with 4HNE and CML antibodies. I have searched through some literature but need a little more time to confirm that these markers will be expressed in certain skin c...

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Thank you for your enquiry and your interest in our products. This antibody is not target specific; it recognizes any proteins which have Carboxymethyl Lysine groups. It may well be that the customers detects several different proteins. I...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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